Effects Of Trioxygen Therapy To Ameliorate Cognitive Dysfunction In Sleep-deprived Rats

Objective Whether trioxygen therapy can improve cognitive function and related mechanisms in rats after sleep deprivation has not been thoroughly investigated.Therefore,this study intends to observe the effect of trioxygen treatment on the cognitive function of sleep-deprived rats and explore the possible mechanisms by using abdominal trioxygen injection.Methods Weight about 300 g of 5 months of middle-aged male rats,SPF,adapt to culture 7 days,make rat sleep deprivation model and randomly divided into 4groups,respectively,group C(no sleep deprivation + intraperitoneal injection of saline),SD group(sleep deprivation + intraperitoneal injection of saline)and SD1,SD2 group(sleep deprivation + intraperitoneal injection of 30,60 ug / ml).The spatial learning ability and memory level and anxiety were analyzed by open field experiment and Morris water maze.The levels of inflammatory cytokine tumor necrosis factor-α(TNF-α),intercytoin-1 β(IL-1 β),interleukin-4(IL-4),interleukin-6(IL-6)and interleukin-10(IL-10)were measured in rat hippocampus after treatment by enzyme-linked immunosorbent assay(ELISA).Rat hippocampal neurons were examined under a high-magnification microscope by using hematoxylin-eosin(HE)staining.Results The results of absent field behavior experiment in sleep deprivation model rats showed that the number of modification,number of entering the central area and total distance in SD group were significantly lower than that of rats in group C(P <0.05),while the number of defecation grains was significantly more than that in group C(P <0.05).In addition,the total distance,number of entering the central area,modification times and number of defecation particles in the SD2 group were also significantly better than that of the SD group(P<0.05),but the difference in the movement distance in the SD1 group was not statistically significant compared with the SD group(P> 0.05).The results of the Morris water maze behavior in the sleep deprivation model showed that the SD group(P <0.05),while the SD1 group and the SD group were not statistically significant(P> 0.05)compared with the SD2 group(P <0.05).Compared with the SD group and C,the number of rats crossing the original platform was significantly reduced and the difference was statistically significant(P <0.05);the SD2 group was significantly higher than the SD group(P <0.05).Enzyme linked immunosorbent assay(ELISA)showed that the content of pro-inflammatory factors TNF-α,IL-1 β and IL-6 in SD group(P <0.05),while SD2 pro-inflammatory factors IL-1 β,IL-6 and TNF-α in SD group(P<0.05),pro-inflammatory factors IL-1 in SD1 rats were not significantly different from SD group(P> 0.05).The content of anti-inflammatory cytokine IL-4 and IL-10 in SD group(P <0.05),anti-inflammatory factors IL-4 and IL-10(P <0.05)in SD1 group and SD2 group,and anti-inflammatory factors IL-4 and IL-10 were significantly lower than those in SD2 group(P <0.05).Under high magnification after HE staining: compared with group C,the neurons in CA1 hippocampus of SD group were incomplete,reduced number,arranged evacuation,disordered distribution,deep cytoplasm,and fixed nucleus;compared with SD group,the neurons in CA1 hippocampus of SD2 group were relatively complete,large number,close arrangement,relatively uniform distribution,and reduced nucleus shrinkage.Conclusions High concentration of medical trioxygen can improve anxiety-and depression-like behavior and reduce the impairment of spatial memory ability in sleep-deprivation model rats.High concentration of medical trioxygen can significantly improve the cognitive dysfunction caused by sleep deprivation in rats,and the related mechanism of this improvement may be related to the significantly lower expression levels of TNF-α,IL-1 β,and IL-6 in the hippocampus.