The Effect Of Shaji Wuwei Powder On Lipid Metabolism And Oxidative Stress Of Atherosclerotic Model Mice

Objective:By observing the effects of Mongolian medicine Shaji Wuwei powder on lipid indexes TG,TC,HDL-C,LDL-C,contents of oxidative stress-related proteins MDA,NO,ET-1,and expressions of Nrf2 and NQO1 in AS model mice,the mechanism of inhibiting atherosclerosis and anti-oxidative stress reaction was explained.To provide experimental basis for preventing and treating AS with Shaji Wuwei powder.Methods:Eight six-week-old male C57BL/6J mice and 26 Apo E^-/-mice weighing 20-22g were selected.After one week of incubation,C57BL/6J mice were used as control group and Apo E^-/-mice as model group.After the control group was fed with basic feed and the model group was fed with high-fat feed for 16 weeks,2 mice in each group were randomly selected to take the aorta tissue for oil red O staining to observe the pathological changes,significant deposition of lipid plaque was observed in the intima of the mouse aorta to demonstrate the successful replication of the arteriosclerosis model.After the establishment of the model,the remaining mice in the model group were randomly divided into the model group（n=6）,the Xuezhikang Group（n=6）,the Shaji Wuwei powder low-dose group（n=6）and the Shaji Wuwei powder high-dose group（n=6）The control group and the model group were given0.1 ml/（10 g·D）normal saline by gavage,Xuezhikang group was given Xuezhikang 2.0mg/（kg·d）,the Shaji Wuwei powder low-dose group was given 0.65mg/（kg·d）,the Shaji Wuwei powder high-dose group was given 2.6 mg/（kg·d）.The samples were taken after 4weeks of continuous administration.The contents of Tg,TC,LDL-C,HDL-C,MDA and NO in serum were detected by biochemical analyzer,the contents of endothelin-1 in serum were detected by Elisa,and the expressions of Nrf2 and NQO1 in aortic tissue were detected by PCR.Results:1.The body mass of mice in each group was measured every two weeks.At the 16th week,the body mass of mice in each group was increased compared with that at the beginning.The mice fed with ordinary feed were in good mental state,with bright fur and flexible activities.The mice fed with high-fat diet had worse mental status and more obesity.At the 17th week,the drug was given and the same feed was given.The cycle of Drug Administration was 4 weeks.2.A mouse model of atherosclerosis（AS）was established.Oil Red O staining of the aorta revealed the formation of arteriosclerosis plaque and lipid deposition in the wall of the aorta.3.Results of serum lipids:the contents of TG,TC,LDL-C in serum of mice fed with high-fat diet were significantly higher than those fed with normal diet,while HDL-C was significantly lower.Compared with the model group,the serum levels of Tg,TC,LDL-C in the low-dose group,high-dose group and positive control group decreased,while HDL-C increased.4.The contents of MDA and NO in serum of mice in each group were compared:Compared with the control group,the MDA content increased and the NO content decreased in the model group,and the difference was statistically significant（P<0.05）.Compared with the model group,the content of MDA decreased and the content of NO increased in the drug group,which was statistically significant（P<0.05）.Compared with the seabuckthorn-5 miso low-dose group:the MDA level and NO level were significantly increased between the positive control group and the seabuckthorn-5 miso high-dose group,and the difference was statistically significant（P<0.05）.There was a significant difference between the high-dose group of sea buckthorn-5 and the active control group（P<0.05）.5.The expression of ET-1 protein in each group:Compared with control group,ET-1protein content in model group was significantly increased（P<0.05）.Compared with model group,the serum ET-1 protein content in administration group was decreased,with statistical significance（P<0.05）.There was statistically significant difference in serum ET-1 content between the high-dose and positive control groups and the low-dose groups of Shaji Wuwei powder（P<0.05）.There was no significant difference between the high-dose group of Shaji Wuwei powder and the positive control group（P>0.05）.6.The expression of NQO1 and NRF2 protein in serum of each group:Compared with the control group,the m RNA expression of NQO1 and Nrf2 in the model group was significantly downregulated（P<0.05）,compared with the model group,the expression of NQO1 and Nr F2 m RNA in the low-dose and high-dose group and the positive control group was significantly upregulated and statistically significant（P<0.05）,and compared with the low-dose group of seabuckthorn-5,the m RNA expression of NQO1 and Nr F2 in the high-dose group and the positive control group was significantly up-regulated（P<0.05）.Conclusion:1.Mongolian medicine Shaji Wuwei powder can regulate lipid metabolism disorders and inhibit the development of atherosclerosis.2.Mongolian medicine Shaji Wuwei powder may play an antioxidant role in preventing atherosclerosis by regulating Nrf2 and its downstream antioxidant gene NQO1.3.Mongolian medicine Shaji Wuwei powder may inhibit the occurrence and development of atherosclerosis by reducing the content of MDA and ET-1 in serum and increasing NO.