Study On Hypoglycemic Constituents Of Ethyl Acetate Part Of Rubus Sachalinensis Leveile

Mongolian Medicine Rubus Sachalinensis Leveile,also known as Raspberry Wood,Bogri Lejigen,and Gandagari,is mainly used in clinical Mongolian medicine to treat diseases such as colds,fever,plague,asthma,lung heat cough,and Heyi fever.Currently,pharmacological studies have shown that Rubus Sachalinensis Leveile have hypoglycemic effects,but their pharmacological components are not yet clear.This article applies chromatography technology to separate chemical components from Rubus Sachalinensis Leveile,and then uses molecular docking and network pharmacology technology to predict the hypoglycemic activity of known compounds in medicinal herbs,providing new basis for the further development and quality control of Rubus Sachalinensis Leveile.This article uses 75% ethanol to extract three times,followed by petroleum ether,ethyl acetate,and n-butanol for extraction.The ethyl acetate site was separated using methods such as forward silica gel column and reverse ODS column,resulting in a total of 16 compounds.The monomer compound structure was determined through nuclear magnetic resonance spectroscopy analysis and literature comparison,and its structural names were Kaempferol-3-O-β-D-glucopyranoside,Stigmasterol-3-O-β-Dglucopyranoside,Sarsasapogenin-3-O-β-galactopyranoside,Lactucine,Betulinic acid,Chrysophanol,Juglanin,Rutin,β-Sitosterol,Hexadecanol,Emodin,Centaureidin,α-D-Glucopyranosid-O-β-D-fructofuranoside,Beta-sitosterol-3-O-β-D-glucopyranoside,liriodendrin,Esculetin.From the 16 monomeric compounds obtained,potential active compounds were screened through network pharmacology.The database was used to screen and intersect the targets of 16 compounds and diabetes respectively.254 intersected targets were obtained and a "drug-component-disease-target" network diagram was established.Compounds 3 and 15 were screened to be the most relevant to diabetes targets,that is,potential active compounds.Through PPI network analysis and core target screening of intersection genes,GSK3 B and AKT1 were selected as the most core targets,and then molecular docking technology was used to dock potential active compounds(compounds 3 and 15)and core targets.The results showed that their docking binding energy was less than-8.5 kcal/mol,showing strong binding energy and stability,It is thus proved that compound 3 and compound 15 have potential pharmacodynamic effects on the treatment of diabetes.Finally,through GO analysis and KEGG enrichment analysis,the results showed that the chemical components isolated from the ethyl acetate part of the Mongolian medicine Rubus Sachalinensis Leveile were related to diabetes related lipids,atherosclerosis,PI3K-AKT signaling pathway,alcoholic liver disease signaling pathway,etc.Therefore,it can be concluded that the chemical composition of Rubus Sachalinensis Leveile is indeed related to the pathogenesis of diabetes,and it is necessary to continue to dig and study deeply,so as to provide a strong argument for the material basis and pharmacological activity of Rubus Sachalinensis Leveile.