| Objective:Explore the relationship between Dectin-1 SNP rs3901532、rs3901533、rs7309123 and disease susceptibility in patients with pityriasis versicolor,as well as the correlation between the disease and IL-17.Methods:There were 60 subjects in the experimental group,including 34 males and 26 females,aged from 10 to 70 years old,with an average age of 29.43±12.49 years old.There were 60 subjects in the control group,including 37 malesand 23 females,aged from 15 to 51 years old,with an average age of 32.93±9.83years old.After collecting whole blood samples,DNA and plasma were extracted.The genotype and allele distribution frequency of rs3901532,rs3901533 and rs7309123 of Dectin-1 gene were detected by polymerase chain reaction(PCR)and gene sequencing.Plasma IL-17 levels were determined by enzyme-linked immunosorbent assay(ELISA).Results:1.SNPs Hardy-Weinberg genetic balance testIn the control group,the genotype of Dectin-1 rs3901532 conforms to Hardy-Weinberg genetic equilibrium law(χ2=0.23,P=0.89),and the genotype of Dectin-1 rs3901533 conforms to Hardy-Weinberg genetic equilibrium law(χ2=0.42,P=0.81),and the genotype of Dectin-1 rs7309123conforms to Hardy-Weinberg genetic equilibrium law(χ2=1.91,P=0.38),indicating that the gene frequency of each point in the sample population had reached the genetic equilibrium state and was representative of the population.2.Comparison of genotypes and allele frequencies of Dectin-1 rs3901532,rs3901533 and rs7309123 between the experimental group and the control group.rs3901532:Genotype analysis of experimental group and control group(χ2=5.425,P=0.077),allele analysis(χ2=1.441,P=0.303),P>0.05 showed that there was no statistical difference in genotype and allele frequency between the two groups.rs3901533:Genotype analysis of experimental group and control group(χ2=1.115,P=0.639),allele analysis(χ2=0.231,P=0.749),P>0.05 showed that there was no statistical difference in genotype and allele frequency between the two groups.rs7309123:Genotype analysis of experimental group and control group(χ2=0.676,P=0.777),allele analysis(χ2=0.025,P=0.875),P>0.05 showed that there was no statistical difference in genotype and allele frequency between the two groups.3.Comparison of genotype and allele frequency of Dectin-1 rs3901532,rs3901533 and rs7309123 in the experimental groupThere was no statistical significance in the distribution of genotype and allele frequency of Dectin-1 rs3901532,rs3901533 and rs7309123 between relapse(initial treatment and retreatment)and different pigmentation types(hyperpigmentation,hypopigmentation,and coexistence of hyperpigmentation and hypopigmentation)(P>0.05).4.IL-17 distribution in plasmaThe median P50 of IL-17 distribution in the experimental group was 3.72 pg/ml and the Quartile spacing Q(P75-P25)was 4.41 pg/ml,while the median P50 of IL-17 distribution in the control group was 3.93 pg/ml and the Quartile spacing Q(P75-P25)was 3.49 pg/ml.There was no significant difference in distribution between the two groups(P>0.05).Conclusion:1.There was no significant difference in genotypes and allele frequencies of Dectin-1 rs3901532,rs3901533 and rs7309123 between the experimental group and the control group,suggesting that the polymorphism of Dectin-1 rs3901532,rs3901533 and rs7309123 may not be related to the susceptibility to pityriasis versicolor.2.There was no significant difference in the genotypes and allele frequencies of Dectin-1 rs3901532,rs3901533 and rs7309123 between the experimental groups with or without recurrence and different pigment types.It is suggested that Dectin-1 rs3901532,rs3901533 and rs7309123 gene polymorphisms are not associated with recurrence and different pigment types in patients with pityriasis versicolor.3.There was no significant difference in IL-17 level between the experimental group and the control group,suggesting that IL-17 may not be involved in the pathogenesis of pityriasis versicolor. |
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