Comparative Evaluation Of The Diagnostic Value Of Two Test Methods For Children With Mycoplasma Pneumoniae Pneumonia In Children

Objective:To explore the diagnostic value of two test methods of fluorescence quantitative PCR method and particle agglutination method in children with different degrees of Mycoplasma pneumoniae pneumonia in Guangyuan area.Methods:Retrospectively analyzed 339 children with suspected Mycoplasma pneumoniae pneumonia admitted to Guangyuan Central Hospital from May 2018 to May 2020,age:March to 14 years old,divided into～1 year old group,1 to 3 year old group,3 to 6 year old group,6～age group,divided into<7 days group,≥ 7 days group according to the course of the disease,respectively,the detection of respiratory tract specimens of Mycoplasma pneumoniae DNA(MP-DNA),Serum specimens in acute and convalescent periods of mycoplasma pneumoniae antibody(MP-Ab),based on the two serum detection antibody titers of more than four times as the standard,the use of t test and χ2 test,according to the sex,age,course,severity of the disease and other aspects of the analysis and summary of the diagnostic effect of single detection and joint testing.Results:1.339 specimens were detected,of which a total of 159 cases of MP infection were judged by the gold standard,with a positive rate of 46.9%,of which 81 cases of male children,the positive detection rate was 46.0%,and 78 cases of female children,the positive detection rate was 47.9%,and the difference between the two was not statistically significant(P>0.05).2.The sensitivity,negative predictive value,positive predictive value,and accuracy of the two methods were 83.01%,86.22%,92.3%,and 91.74%,all of which were better than those detected by a single method(P<0.05);among them,the accuracy of MP-DNA detection of respiratory samples was 73.74%better than that of serum MP-Ab detection of 67.55%,but there was no statistical difference in results(P>0.05).3.For the particle gel method to detect the positive detection rate of serum antibody Mycoplasma pneumoniae with age,The course of the disease was on the rise(P<0.05),but the difference in the positive detection rate of fluorescence quantitative PCR method in age and course was not statistically significant(P>0.05).4.The positive detection rate of fluorescence quantitative PCR in infant and young children was higher than that of particle agglutination method,and the difference was statistically significant(P<0.05).5.The results of the detection of two methods alone and the joint test showed that the positive rate of the severe disease group was higher than that of the non-severe group,and the difference was statistically significant(P<0.05).Conclusion:For children with mycoplasma pneumoniae pneumonia in Guangyuan area,the combined detection accuracy of MP-DNA in respiratory tract samples and serum MP-Ab was higher.The positive rate of MP infection detected by serum MP-Ab showed an increasing trend with the increase of age and the prolonging of disease course.MPP was more likely to occur in the early school age and school age.For infants,the positive rate of fluorescence quantitative PCR was higher than that of particle agglutination.The more serious the MP infection,the higher the positive rate of particle agglutination and fluorescence quantitative PCR.