| Objective:Ischemic stroke(IS)is one of the major diseases that harm human health.It’s characterized by high morbidity,mortality,disability and recurrence.The pathogenesis of IS is influenced by multiple genetic and environmental factors.microRNA cluster(miRNA cluster)refers to two or more closely related miRNA on the chromosome,mainly involved in the co-regulation of gene expression.Previous studies have suggested that miRNA clusters may be involved in the pathophysiology of IS.The aim of the study was to detect the expression of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster in patients with ischemic stroke(experimental group)and healthy people(control group).At the same time,the difference of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster expression between experimental group and control group was analyzed,and the correlation between miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster and IS was analyzed as well,to provide theoretical evidence for the prevention and treatment of ischemia-reperfusion injury in IS.Methods:A total of 83 inpatients(experimental group)diagnosed as IS were collected in the department of neurology of the affiliated hospital of north sichuan medical college from July 2020 to December 2020,and 50 healthy subjects(control group)were examined during the same period.The informationsincluding crowd characteristics,history of smoking,history of drinking,course of disease,NIHSS score,TOAST type,length of stay,complications and other related clinical data were recorded.Meanwhile,peripheral blood mononuclear cells were extracted from peripheral blood by density graditrnt centrifugation.the expression of miRNA-338-3p,miRNA-1250-5p,and miRNA-3065-5p were detected by adsorption of centrifugr mononuclear cells,reverse transcription,and Real-time quantitative Reverse Transcription Polymerase Chain Reaction(RT-qPCR)in peripheral blood mononuclear cells.The correlation among miRNA-338-3p,miRNA-1250-5p,and miRNA-3065-5p between experimental group and control group,and subgroup analyses were made using IBM SPSS 25.0.Results:(1)Comparison of the basic characteristics and stroke risk factors between the experimental group and the control group:There was no statistical significance in age and gender between the experimental group and the control group(P>0.05).The stroke risk factors of hypertension,diabetes,heart disease,arteriosclerosis,smoking history,history of stroke in the experimental group were higher than those in the control group(P<0.05).No significant statistical difference was found in the atrial fibrillation,dyslipidemia,history of drinking(P>0.05).Analysis of disease severity and types subgroup in experimental group:The patients were divided into mild stroke(51.8%,43 cases)and non-mild stroke(48.2%,40 cases)according to admission NIHSS score.The length of hospitalization,discharge NIHSS score and discharge MRS score of mild stroke were significantly lower than those of non-mild stroke(P<0.001).There was no significant difference in the course of disease between the two groups(P>0.05).According to the TOAST classification,SAA(44.6%,37 cases)and LAA(39.8%,33 cases)were most common,followed by CE(13.3%,11 cases),SOE(2.4%,2 cases)and SUE(0 cases).NIHSS scores on admission,NIHSS scores on discharge and mRS Scores on discharge of LAA type were significantly higher than those of type SAA;NIHSS scores on admission and mRS scores on discharge of CE type were significantly higher than those of type SAA(P<0.05).The hospitalization time of LAA(21.1 ±13.6)was longer than that of SAA(12.7±4.4)(P<0.05).There was no significant difference in the duration of disease among the subgroups(P>0.05).Laboratory index between the experimental group and the control group:The white blood cell count and the percentage of neutrophil in the experimental group were significantly higher than those in the control group(P<0.05).The percentage of mononuclear cells and lymphocyte,hemoglobin,red blood cell count,and high-density lipoprotein in the experimental group were lower than those in the control group(P<0.05)。There were no significant differences in platelet count,uric acid,triglyceride,total cholesterol and LDL-C between the two groups(P>0.05).(2)Expression of miRNA-338-3p/miRNA-1250-5p/miRNA-30655pcluster in experimental group and control group:The expression of miRNA-1250-5p in experimental group(2.04±0.22)was higher than that in control group(1.54±0.33)(P=0.002).The expression of miRNA-3065-5p in experimental group(6.41±2.17)was higher than that in control group(1.42±0.24)(P<0.001).The difference of miRNA-338-3p expression was not statistically significant in the experimental group(1.87±0.22)and the control group(1.25±0.11)(P=0.309).(3)The expression of miRNA-338-3p/miRNA-1250-5p/miRNA-30655p cluster in IS subgroup:The expression of miRNA-3065-5p in mild stroke group(6.9±26.2)was higher than that in non-mild stroke group(5.9±9.1)(P<0.05).At the same time,miRNA-3065-5p in the disabled group(7.0 ±10.3)was higher than that in the good prognosis group(6.1± 23.6)(P<0.001).The difference of miRNA-338-3p and miRNA-3065-5p in NIHSS sub-group after discharge was statistically significant(P<0.05).The expression of miRNA-338-3p was lower(1.6± 1.9)in the NIHSS ≤3 sub-group than in the high NIHSS score sub-group(2.4± 2.1);and the expression of miRNA-3065-5p was higher(6.6± 23.9)in the lower sub-group than in the high sub-group(6.1±9.8).In addition,miRNA-338-3p expression in IS patients with complications(2.4± 2.4)was higher than that in patients with no complications(1.5± 1.6)(P<0.05).The expression of miRNA1250-5p was significantly different in different stages of the disease(P<0.05).(4)Correlation analysis between miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster and IS:There was a significant positive correlation among miRNA-338-3p,miRNA-1250-5p,and miRNA-3065-5pin IS(P<0.001).The expression of miRNA-1250-5p and miRNA-3065-5p was positively correlated with the occurrence of acute IS events(P<0.01).The expression of miRNA-3065-5p was positively correlated with admission NIHSS score and length of stay in patients with IS(P<0.05).The expression of miRNA-338-3p and miRNA-3065-5p was positively correlated with discharge mRS(P<0.05).miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster was positively correlated with the neutrophil percentage and negatively correlated with the lymphocyte percentage(P<0.05).There was no significant correlation between miRNA-338-3p/miRNA-1250-5p/miRNA3065-5p cluster and age,sex,course of disease,TOAST type,complication,discharge NIHSS score,red blood cell count,hemoglobinand blood lipid(P>0.05).Conclusions:miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster might be involved in the pathophysiological process of ischemic stroke.miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster was closely related to IS inflammation index,disease severity score and prognosis index,which suggested that miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p cluster might influence IS severity and prognosis by regulating inflammatory response. |
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