Research On The Correlation Between IL-19 And CD4+/CD8+T Cells In Acute Secretory Otitis Media

Objective:Acute secretory otitis media,as a common multiple disease in otorhinolaryngology,head and neck surgery,has seasonal characteristics and is common in children aged 2-5 years old.it is one of the common causes of hearing loss in children.children are often ignored because they can not accurately express their clinical symptoms,so their potential health hazards should not be underestimated.At present,experts and scholars have conducted a large number of clinical trials and studies on acute secretory otitis media,including immune response and gene-related studies,but the mechanism related to the occurrence and development of the disease is still controversial and needs to be improved.Therefore,this paper studies the correlation between interleukin-19(IL-19)and the ratio of CD4+/CD8+T cells in peripheral blood and patients’ hearing loss in the course of acute secretory otitis media,to explore the clinical relationship between IL-19 and acute secretory otitis media,and to provide new ideas for disease immune-related mechanism and clinical diagnosis and treatment.Methods:The patients with acute secretory otitis media diagnosed in 404 Hospital of Mianyang,Sichuan Province from December 2020 to November 2021 were selected in the observation group,and the healthy outpatients in the control group were selected in the same period.The middle ear effusion and peripheral blood of the observation group and the peripheral blood of the control group were collected,and the content of IL-19 in middle ear effusion and peripheral blood was detected by ELISA method.The proportion and ratio of CD4+ and CD8+T cells in peripheral blood of the two groups were analyzed by flow cytometry.T-test and Pearson correlation analysis were used to test the difference and correlation between the data of each group.Results:There was no significant difference in general clinical data between the observation group and the control group.CD4+,CD8+T cells and CD4+/CD8+ratio in the observation group were higher than those in the control group,and there were significant differences between the two groups.The average level of IL-19 in middle ear effusion in the observation group(50.46±26.48/pg/ml)was higher than that in the observation group(32.80±22.63/pg/ml)and the control group(11.12±9.25/pg/ml).There was significant difference among the three groups(P<0.05).Pearson analysis was used to analyze the correlation between the content of IL-19 in middle ear effusion and peripheral blood and the ratio of CD4+/CD8+T cells in peripheral blood in the observation group.The results showed that the content of IL-19 in middle ear effusion and peripheral blood was positively correlated with the ratio of CD4+/CD8+T cells in peripheral blood(r middle ear effusion=0.737,rperipheral blood=0.773,P<0.001).Conclusion:The content of IL-19 in middle ear effusion in the observation group was higher than that in the peripheral blood of the observation group and that of the control group.There was a significant correlation between IL-19 and CD4+/CD8+T cell ratio in middle ear effusion and peripheral blood in patients with secretory otitis media.It is involved in the inflammatory process of acute secretory otitis media.The level of IL-19 in peripheral blood and middle ear effusion can reflect the inflammatory degree of secretory otitis media to some extent.