| Objective Analysis of clinical characteristics of infants with Infant Dyschezia,and to explore the factors related to the occurrence of Infant Dyschezia in infants;The 16SrDNA method was used for meconium sequencing analysis to understand the richness,diversity and composition of intestinal flora in meconium of infants with Infant Dyschezia.To provide ideas and theoretical basis for the prevention and treatment of Infant Dyschezia.Methods Newborns born in Shenzhen Maternal and Child Healthcare Hospital from January 2021 to April 2021 were selected,meconium was collected for 72 hours,and the basic clinical data of infants and mothers,such as infant gender,birth weight,gestational age,were collected.,mode of delivery,presence or absence of premature rupture of membranes,amniotic fluid status,history of antibiotic use,postnatal feeding methods and maternal age,education level,economic level,family history of allergies,history of underlying diseases during pregnancy(Gestational diabetes mellitus,Gestational hypertension,Hypothyroidism,hyperthyroidism,Intrahepatic cholestasis,etc.),history of prenatal antibiotic use,weight gain during pregnancy,etc.The enrolled subjects were followed up by telephone at 3 months of age,and filled out a questionnaire.The content of the questionnaire covered growth and development,dietary patterns,gastrointestinal symptoms,and stool conditions.According to the Rome Ⅳ diagnostic criteria,the infants diagnosed with Infant Dyschezia were selected as the Infant Dyschezia group,the rest were non-Infants Dyschezia group,compare the differences in basic clinical information between the infants with Infant Dyschezia group and the non-Infant Dyschezia group and analyze the related factors;using the propensity score matching method,select the same number of cases in the non-Infant Dyschezia group as healthy controls at a ratio of 1:1,16S rDNA analysis was performed on the two groups of meconium,analyzed the diversity,richness and composition of meconium intestinal flora of two groups.Results 1.A total of 505 subjects were included in the study,and 20 infants with confirmed Infant Dyschezia,444 infants without infant Dyschezia,41 lost to follow-up,There were significant differences in maternal education level,family history of allergies and prophylactic antibiotic use in the neonatal period between the infants with Infant Dyschezia group and the non-Infant Dyschezia group.(P<0.05).Higher maternal education was a protective factor for infants with Infant Dyschezia.Family history of allergy,prophylactic use of antibiotics in the neonatal period were the Risk factors for infants.2.There was no significant difference in the diversity and richness of the meconium intestinal flora between the infants with Infant Dyschezia group and the healthy control group;there were statistically significant differences in the composition of phylum,class,order,family and genus(P<0.05).3.The prophylactic use of antibiotics in the neonatal period compared with no prophylactic use of antibiotics in the neonatal period was statistically different in Enterobacter,Parabacter and Ruminococcus(P<0.05).Conclusions 1.The higher the mother’s education level,the lower the risk of Infant Dyschezia,and the increased risk of Infant Dyschezia with family history of allergies,neonatal prophylactic antibiotics;2.There was no difference in the richness and diversity of the Meconium intestinal flora in Infant Dyschezia and non-Infant Dyschezia,but there were differences in the composition structure,suggesting that the difference in the composition and structure of the early intestinal flora may be related to the occurrence of Infant Dyschezia;3.The prophylactic use of antibiotics in the neonatal period decreased the abundance of Enterobacter and Ruminococcus,while the abundance of Parabacteroides increased,suggesting that the prophylactic use of antibiotics in the neonatal period may increase the risk of having Infant Dyschezia by change the abundance of meconium intestinal flora in infants. |
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