Detection Of Autophagy Of Guinea Pigs Infected With Varicella-zoster Virus And Related Research

BackgroundVaricella-Zoster Virus(VZV)is a typical human herpes virus with natural susceptibility to skin,nerves and lymphocytes.It can cause chickenpox after initial infection,and then latent in dorsal root ganglia,where reactivation can lead to shingles.At present,the research on the latent infection and reactivation mechanism of VZV has not been conclusive.Autophagy is generally regarded as a means of body self-protection to degrade abnormal substances or foreign substances in cells.Some studies have explored the relationship between herpes virus and autophagy in herpes zoster skin lesions and nerve-derived cells.ObjectiveThe dorsal root ganglia of guinea pigs were infected with VZV to establish a VZV in vivo infection system.The aim was to clarify the relationship between VZV infection and autophagy at the animal level and confirmed the occurrence of autophagy in guinea pig dorsal ganglion neurons.As well as the biological function and elucidation of the mechanism of autophagy during VZV infection.Methods1.The guinea pigs in the infection group were injected with VZV-infected mononuclear cells intravenously to establish an infection model,while the control group was injected with the same amount of VZV-infected mononuclear cells.After 14 days,peripheral blood was collected and guinea pigs were dissected to obtain dorsal root ganglion tissue.VZV DNA in blood was detected by PCR method,VZV virus particles in DRG tissue were observed by transmission electron microscope,and VZV-related proteins were detected by Western blot and immunohistochemistry.2.Take 32 normal-grade 4-6 weeks old female Hartley guinea pigs,and take 8 untreated guinea pigs as blank control group,and randomly divide 24 virus-infected guinea pigs into 3 groups according to whether autophagy intervention is performed or not.8 animals:no treatment group,autophagy induction(RM)group and autophagy inhibition(3-MA)group.After 14 days,the dorsal root ganglia(DRG)of guinea pigs were dissected,the morphology of virus particles and the morphology and number of autophagosomes were observed by transmission electron microscope(TEM),and the expressions of virus-related proteins and autophagy proteins were detected by Western blot.Results1.Guinea pigs were infected by intravenous injection of VZV-infected mononuclear cells.After 14 days,VZV DNA could be detected in the peripheral blood of the guinea pigs,immature viral particles were observed in the dorsal root ganglion tissue,and the expression of the corresponding VZV protein was detected.2.The occurrence of autophagy was confirmed in the dorsal root ganglia of guinea pigs.After treatment with autophagy intervention drugs,it was found that the expression of VZV-related proteins was significantly inhibited in the autophagy-inhibited group compared with the no-drug treatment group,while the autophagy-induced group was significantly inhibited.VZV protein expression showed no significant difference.Conclusion1.Intravenous injection of VZV-infected guinea pig mononuclear cells can construct a VZV-DRG guinea pig infection model.2.It is confirmed that autophagy occurs after VZV infection of guinea pig DRG.After inhibiting the level of autophagy,the expression of viral structural and functional proteins decreased,thereby affecting viral biosynthesis,which provides a new method for the prevention and treatment of neurological symptoms related to varicella-zoster virus infection.potential therapeutic targets.