Effects Of Ferulic Acid On Proliferation,Differentiation And Expression Of Inflammatory Factors In MC3T3-E1 Cells Cultured With High Glucose

Background and ObjectiveDiabetes Mellitus(DM)is a chronic human metabolic disease characterized by a chronically high level of glycemic index,mainly caused by impaired insulin secretion activity.Diabetes mellitus impairs the metabolic ability of the body and leads to a series of complications.Diabetic Osteoporosis(DOP)is a common chronic complication of diabetes mellitus,characterized by hyperglycemia,bone loss,increased bone fragility and minor fractures,which severely affects the life quality of patients and has a high disability rate,because DM adds to the medical and financial burden of the world,and the research mitigation and treatment means have aroused great attention.Ferulic Acid(FA)is a kind of hydroxycinnamic acids found in plant cell walls,which has multiple pharmacological activities and is widely used in the prevention of cardiovascular disease,diabetes and cancer.Studies have shown that ferulic acid has strong antioxidant therapeutic activity,can inhibit osteoclast formation and stimulate osteoblast function,which may be beneficial in the treatment or prevention of diabetic osteoporosis.However,the mechanism of how ferulic acid affects osteocytes and osteoblasts is not yet clear,so in this study we conducted to investigate whether ferulic acid can relieve the damage in osteoblasts cultured with high glucose and quest the effect of ferulic acid on high glucose cultured osteoblasts,to provide a basis for future clinical application of the drug.Methods1.To quest the useful high glucose concentration range that can inhibit the proliferation and osteogenic differentiation of MC3T3-E1 osteoblasts in vitro.In this study,MC3T3-E1 cells were cultured in different concentration sugar containing medium.The proliferation of MC3T3-E1 cells,the expression of inflammatory factors and the concentration of intracellular Reactive Oxygen Species(ROS)were detected by Cell Counting Kit-8(CCK-8),Enzyme Linked Immunosorbent Assay(ELISA)and flow cytometry to determine the changes in proliferation ability of osteoblasts.The changes of osteogenic ability were detected by Alkaline Phosphatase(ALP)activity and Alizarin Red S Staining(ARS).2.To detect the ideal concentration of ferulic acid to promote the proliferation and calcification of MC3T3-E1 osteoblasts under high glucose conditions in vitro.In this study,MC3T3-E1 osteoblasts were co-cultured by mixing ferulic acid with certain concentration of high glucose medium.The proliferation of MC3T3-E1 osteoblasts was detected by CCK-8,the expression of inflammatory factors by ELISA,alkaline phosphatase activity by ALP,and ROS by flow cytometry.Results1.At the concentration of 50 mmol/L sugar,the cell proliferation ability and ALP activity decreased significantly,the formation of mineralized nodules decreased,the expression of inflammatory factors increased significantly,and the expression of intracellular ROS increased(P<0.05).2.The cell proliferation capacity tended to increase with increasing concentrations of ferulic acid of 0.1-10 μmol/L,the synthesis and secretion of ALP increased,the osteogenic differentiation was enhanced,and the expression of inflammatory factors and intracellular ROS were inhibited(P<0.05).ConclusionIn this study,we found that ferulic acid could reduce MC3T3-E1 cell injury induced by high glucose stimulation.