| Objective:To explore the clinical characteristics of type 2 diabetes mellitus complicated with non-alcoholic fatty liver and the differential characteristics of intestinal flora.Methods:This study collected patients who were suffered from type 2 diabetes and hospitalized in the Department of Endocrinology and Metabolism,Pearl River Hospital,Southern Medical University Hospital,and divided into non-alcoholic fatty liver group with type 2 diabetes and type 2 diabetes without fatty liver according to the results of B ultrasound or computed x-ray tomography（CT）.Collect the patient’s general information height,weight,age,gender,etc.,and collect the patient’s blood sample to measure biochemical indicators.Sequencing of stool samples using the 16srDNA sequencing method.Results:This study included 72 patients with type 2 diabetes mellitus who were non-alcoholic fatty liver disease and 63 patients with type 2 diabetes without fatty liver.Univariate analysis revealed statistically significant differences in the course,glycosylated hemoglobin,weight,fasting C-peptide,total cholesterol（TC）,serum alanine transferase（ALT）,total bilirubin,triglycerides（TG）,direct bilirubin,indirect bilirubin,low-density lipoprotein（LDL）,glutamyl transpeptidase（GGT）,albumin,urine creatinine,serum creatinine,sodium blood,blood chlorine,and urine protein/creatinine ratio（ACR）between the two groups（P-value was less than 0.05）.Logistic regression analysis was carried out using non-alcoholic fatty liver disease as the dependent variable and the above statistically significant clinical indicators as the independent variable.Logistic regression suggests that weight and triglycerides are risk factors for non-alcoholic fatty liver disease in diabetic patients（P-values are less than 0.05）.The study included 18 type 2 diabetes patients who suffered from non-alcoholic fatty liver disease and 9 patients with type 2 diabetes without fatty liver disease,with no difference in age and sex.16SrDNA sequencing of their fecal specimens yielded species（OTUS）.Beta diversity index analysis suggested that the differences in flora were significant between the two groups,with more flora diversity（P-value less than 0.05）in the group with type 2 diabetes mellitus and non-alcoholic fatty liver disease.In addition,a linear discriminant analysis（LEfse analysis）was used to obtain statistically significantly different flora（P-value less than 0.05）.The results indicated that compared with the type 2 diabetes without fatty liver group,the abundance of Stenothephomonas,Xanthomonadacea,Xanthomonadales,Ruminococcussp5I39BFAA,Blautia,and Lachnospiraceae increased in the type 2 diabetes group with non-alcoholic fatty liver group,while the Ruminococcaceae and Faecalibacterium were reduced.Conclusion:Weight and triglycerides are risk factors for non-alcoholic fatty liver disease in patients with type 2 diabetes.There were differences in the intestinal flora of the non-alcoholic fatty liver group with type 2 diabetes and the group without fatty liver disease with type 2 diabetes.The study has important implications for the prevention and treatment of non-alcoholic fatty liver disease in type 2 diabetes. |
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