Study On The Mechanism Of Raspberry Safflower On Regulating Sex Hormones,Sexual Function And NO-cGMP Signaling Pathway In Diabetic Sexual Dysfunction Rats

Objective:To investigate the mechanism of raspberry safflower on regulating sex hormones,sexual function and NO-c GMP signaling pathway in diabetic sexual dysfunction rats.Methods:100 6-week-old male SD rats were selected.10 animals were randomly selected as blank group.The DIED rat model was established with STZ and APO.Rats with successful modeling were divided into model group,raspberry group,raspberry-safflower drug pair group,safflower group,and tadalafil group,with 10 rats in each group.The raspberry group,the raspberry-safflower drug pair group,the safflower group,and the tadalafil group were given 1.07 g·kg^-1,1.32 g·kg^-1,0.25g·kg^-1,and 0.52 mg·kg^-1,respectively.Oral gavage,once a day,for 30 days.The body weight of the rats in each group was measured before and after modeling and before and after treatment.At the end of the gavage,the random blood sugar of the rats in each group was measured,the average carotid artery pressure（MAP）of the rats was measured,and the intracavernous pressure（ICP）and ICP/MAP were measured by electrical stimulation of the rat cavernosal nerve.Testosterone,luteinizing hormone and follicle-stimulating hormone in peripheral blood were detected by ELISA.Serum triglyceride and total cholesterol were determined by colorimetric method.The pathological changes of testis were observed by HE staining.The levels of e NOS and n NOS m MRA in the corpus cavernosum were detected by Real-time PCR.Western Blot was used to detect the expression of e NOS,n NOS,i NOS and VEGF protein in corpus cavernosum shuiip..Results:After modeling,the body weight of the rats in the blank group increased（P<0.001）,while the body weight of the model group and the raspberry safflower drug pair group decreased significantly（P<0.001）.After treatment,the body weight of the rats in the blank group increased（P<0.001）,the body weight of the rats in the model group was further reduced compared with that before treatment（P<0.001）,and the body weight of the raspberry safflower drug treatment group was increased compared with that before treatment（P<0.001）,and The body weight of the potted safflower group was higher than that of the model group（P<0.001）.After treatment,the blood sugar of the model group was significantly higher than that of the blank group（P<0.001）,and the blood sugar of the raspberry safflower drug pair group was lower than that of the model group（P<0.001）.The ICP and ICP/MAP of rats in the model group were lower than those in the blank group（P<0.001）,and the ICP and ICP/MAP in the raspberry safflower drug pair group were higher than those in the model group（P<0.01）.There was no difference in MAP among the groups of rats（P>0.05）.The levels of T,NOS and c GMP in the model group were lower than those in the blank group（P<0.001）,while the levels of T,NOS and c GMP in the raspberry safflower drug pair group were higher than those in the model group（P<0.05）.The testicular seminiferous tubules of the rats in the model group atrophied,with irregular shape,loose arrangement,widened gaps,and a large decrease in the number of spermatogenic cells.Compared with the model group,the lesions of seminiferous tubules and spermatogenic cells in the raspberry safflower group were significantly reduced.The levels of e NOS and n NOS m RNA in the raspberry safflower pair group were higher than those in the model group（P<0.05）.The protein expression levels of n NOS,e NOS,i NOS and VEGF in the raspberry safflower group were significantly higher than those in the model group（P<0.01）.Conclusion:Raspberry safflower can improve sex hormones and regulate sexual function in patients with DIED,and its mechanism may be related to increasing testosterone,reducing blood sugar and lipids,improving testicular tissue lesions,increasing e NOS,n NOS m RNA expression and n NOS,e NOS,i NOS,VEGF protein expression.expression related.