Revolution CT On Myocardial Perfusion In Rats Under Chronic Low Pressure And Hypoxic Environment At Plateau

Objective: To apply the Revolution CT myocardial perfusion imaging technique to analyze the alteration of myocardial perfusion in rats under the natural environment of the plateau.Methods: Twenty-four male SD rats were randomly divided into 12 rats in the plateau group and 12 rats in the plains group,and were kept in different environments for 30 weeks simultaneously.The area was set at 0.1 mm2,and the values of myocardial blood flow(BF),myocardial blood volume(BV),mean time to passage(MTT),time to peak(TTP),and surface permeability(PS)perfusion parameters were measured,and the differences of each parameter were compared.Blood-related indexes were collected from rats in both groups,and after performing myocardial tissue sampling,HE staining,Masson staining and EPO and VEGF immunohistochemistry were performed to observe the pathological changes and the expression of EPO and VEGF.Results1.The body weight,red blood cells(RBC),hemoglobin(HGB)and red blood cell pressure(HCT)of the rats in the plateau group were higher than those in the plain group(P<0.05),and the platelet(PLT)values in the plateau group were lower than those in the plain group(P<0.05).2.The BV,MTT,TTP and PS values of rats in the plateau group were higher than those in the plain group(P<0.05),and the BF values of rats in the plateau group were lower than those in the plain group(P<0.05).3.Pathological results: The ventricles of the rats in the plateau group were obviously thickened,more collagen fibers were deposited in the myocardial space,the myocardial space was slightly widened,and the myocardial cells were irregularly arranged and some fat vacuoles were formed.4.Immunohistochemical method was used to determine the high expression of EPO and VEGF in myocardial tissue of plateau group compared with plain group.Conclusion1.The heart is in a state of under-perfusion under the natural environment of chronic hypoxia in the plateau,and the myocardium is at risk of ischemia.2.The histopathological changes of myocardium and high expression of EPO and VEGF in rats in the plateau group explain the imaging changes from a macroscopic perspective and provide a basis for plateau disease imaging.