The Role And Mechanism Of CK2/DBC1/SIRT1/P53 Signaling Pathway In Endometrial Carcinoma

Objective By detecting the expressions of CK2,DBC1,SIRT1 and P53 in endometrial carcinoma,the mechanism of CK2/DBC1/SIRT1/P53 signaling pathway in the pathogenesis of endometrial carcinoma was discussed,which provided a new research idea for targeted therapy of endometrial carcinoma.Methods From January 2018 to December 2020,60 cases of endometrial carcinoma diagnosed pathologically in Qingdao University Women and Children’s Hospital were collected(experimental group),and 40 cases of normal endometrial tissue diagnosed pathologically by total hysterectomy due to hysteromyoma or adenomyosis(control group)were taken as the research objects.Immunohistochemical staining(IHC)was used to detect the positive expression rates of CK2α,DBC1,SIRT1 and P53 in the two groups.The expressions of CK2α,DBC1,SIRT1 and P53 were detected by western blotting.The m RNA expression levels of CK2,DBC1 and SIRT1 were detected by real-time quantitative polymerase chain reaction(q RT-PCR).Immunoprecipitation(Co-IP)method was used to detect the changes of the interaction between DBC1 and SIRT1 in the two groups of tissues.Results 1.The expressions of DBC1 and SIRT1 are related to the clinical stage,pathological grade,depth of myometrial invasion and lymph node metastasis of endometrial carcinoma(P < 0.05),but not to the age of patients;2.IHC results showed that the positive expression rates of DBC1 and SIRT1 in the experimental group were 70% and 65% respectively,while those in the control group were 37.5% and 30% respectively,with significant statistical difference(P< 0.05);the positive expression rate of P53 protein was 33% in the experimental group and 62.5% in the control group,the positive expression rate in the experimental group was significantly lower than that in the control group,and the difference was statistically significant(P < 0.05);the positive expression rates of CK2α protein in the experimental group and the control group were 45% and 55% respectively,and there was no significant difference(P > 0.05);3.Western blotting results showed that the expression of DBC1 and SIRT1 in the experimental group was significantly higher than that in the control group;while the expression of P53 in the experimental group was significantly lower than that in the control group,and the difference was statistically significant(P < 0.05);there was no significant difference in the expression of CK2α between the two groups(P > 0.05);4.The results of qRT-PCR showed that there was no significant difference in the m RNA levels of CK2,SIRT1 and DBC1 between the two groups(P > 0.05);5.Co-IP results showed that the protein interaction ability of DBC1 and SIRT1 in the experimental group was lower than that in the control group(P < 0.05);6.Spearman grade correlation analysis showed that there was a positive correlation between DBC1 and SIRT1(r=0.358,P < 0.05).Conclusion 1.DBC1 and SIRT1 may be closely related to the occurrence and development of endometrial cancer,and they may become new therapeutic targets for endometrial cancer;2.The regulation of CK2,SIRT1 and DBC1 in endometrial carcinoma is related to post-translational modification,thus participating in the regulation of various biological processes of endometrial carcinoma;3.There is regulation of the interaction between DBC1 and SIRT1 in endometrial carcinoma,which may be related to the poor prognosis and tumor progression;4.CK2/DBC1/SIRT1/P53 signaling pathway may be regulated in endometrial carcinoma,which may be closely related to the occurrence and development of endometrial carcinoma.