| Objective:This study was to analyze the expression of CXCL8,CXCR1 and CXCR2 in breast cancer tissues before and after neoadjuvant chemotherapy,and their relationship with clinicopathological features,Miller-Payne classification and disease free survival.Methods:From the medical record system and pathological database of breast surgery of the Affiliated Hospital of Qingdao University,62 patients who underwent radical surgery after full course of neoadjuvant chemotherapy were selected.The operation time was from January 2016 to December 2018.The whole process of diagnosis and treatment was treated in our hospital with complete data.Before conducting chemotherapy,the breast needle biopsy was performed on the breast and axillary lesions.The chemotherapy regimen was TAC×6 or AC×4-T×4.2～3 weeks after chemotherapy,modified radical mastectomy or breast conserving surgery for breast cancer were performed.The paraffin sections of patients’ puncture and postoperative pathology were obtained.Immunohistochemical technique was used to detect the pathological tissues after puncture and operation.The percentage of positive cells and staining intensity of CXCL8,CXCR1 and CXCR2 in sections were analyzed,and semi quantitative scores were made.Combined with medical records,the expression of CXCL8,CXCR1 and CXCR2 in breast cancer tissues before and after neoadjuvant chemotherapy was analyzed by SPSS 21 software,and the relationship with the clinicopathological features,Miller-Payne classification and DFS after neoadjuvant chemotherapy was also discussed.Results:1.Clinicopathological characteristics of the breast cancer patients: the median age of onset of the 62 breast cancer patients was 50 years(range,34-66 years);31(50%)patients were not menopausal and 31(50%)were already menopausal at the time of initial diagnosis;5(8.1%)patients were clinical stage I,30(48.4%)stage II and 27(43.5%)stage III;molecular classification of breast cancer,38 patients were Luminal type,16 patients were HER-2-positive type and 8 patients were triple negative type;There were 13patients(21.0%)with Miller-Payne grade G4-G5 after chemotherapy.2.The changes of CXCL8,CXCR1 and CXCR2 in neoadjuvant chemotherapy in breast cancer tissues: the positive rate of CXCL8 after neoadjuvant chemotherapy(41.9%)was higher than that before chemotherapy(27.4%),(P=0.022).The expression scores of CXCR1 and CXCR2 after neoadjuvant chemotherapy were significantly lower than those before chemotherapy(P<0.001).No matter before or after neoadjuvant chemotherapy,the expression scores of CXCR1 and CXCR2 in CXCL8 positive group were higher than those in CXCL8 negative group(P<0.001).3.The relationship between CXCL8,CXCR1 and CXCR2 and the clinicopathological characteristics of patients: the expression of CXCL8 in breast cancer tissues before and after neoadjuvant chemotherapy was related to the N stage,ER/PR status and HER-2 status(P<0.05).The expression scores of CXCR1 and CXCR2 before neoadjuvant chemotherapy were correlated with tumor N stage,ER/PR status and HER-2status(all P<0.05);After chemotherapy,the expression of CXCR1 and CXCR2 was still correlated with ER/PR status and N stage(P<0.05).4.The expression of CXCL8,CXCR1 and CXCR2 in breast cancer before neoadjuvant chemotherapy was not significantly correlated with Miller-Payne classification(P>0.05).The expression of CXCL8,CXCR1 and CXCR2 in cancer tissues after neoadjuvant chemotherapy was correlated with Miller-Payne grade(P<0.05).The Miller-Payne grade in CXCL8 negative group,CXCR1 low expression group and CXCR2 low expression integral group was higher and the chemotherapy response was better.The expression level of CXCR1 after neoadjuvant chemotherapy was the influencing factor of Miller Payne grade(P=0.015,OR=0.069).5.Correlation between pathologic and clinical characteristics and prognosis of patients: tumor T stage,N stage,HER-2 status and The Miller-Payne grade was associated with patient DFS(P=0.001,P=0.022,P=0.012,P=0.042),and patients with T1-T2,N0-N1,HER-2 negative,and Miller-Payne grade G4-G5 had a more favorable DFS than those without.On multivariate Cox regression survival analysis,T stage(P=0.048,HR=2.620)was an independent risk factor for DFS.6.The relationship between CXCL8,CXCR1 and CXCR2 and DFS in breast cancer tissues: the expression of CXCL8 was correlated with DFS before and after neoadjuvant chemotherapy(P<0.01),and DFS in CXCL8 negative group was better.After neoadjuvant chemotherapy,the expression scores of CXCR1 and CXCR2 were correlated with DFS(P<0.05),and the patients with low expression scores of CXCR1 and CXCR2 had better DFS.In Cox regression multivariate survival analysis,the expression of CXCL8 after neoadjuvant chemotherapy was an independent risk factor for DFS(P=0.004,HR=26.990).Conclusions:Neoadjuvant chemotherapy can change the expression of CXCL8,CXCR1 and CXCR2 in the tissue microenvironment of breast cancer.CXCL8,CXCR1 and CXCR2 after neoadjuvant chemotherapy can be used as reference indexes to evaluate the efficacy and prognosis of chemotherapy. |
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