| Purpose Atractylenolide I is the main active ingredient extracted from Chinese herbal medicine Atractylodes.This study observed its effects on intestinal flora,short chain fatty acids and 5-hydroxytryptamine(5-HT)in rats to explore whether it plays a therapeutic role on slow transit constipation(STC)model rats induced by Compound Diphenoxylate Tablets(CDT)by regulating intestinal flora and promoting intestinal motility,so as to provide an effective treatment direction for the clinical application of Atractylenolide I in STC.Methods Forty SD rats were randomly divided into normal group(Group N),model group(Group M),Atractylenolide I group(Group A)and Prucalopride Succinate Tablets group(Group P),with 10 rats in each group.Group M、P and A were treated with CDT suspension at the dose of 15 mg /(kg·d)for 3 weeks.Rats in Group N were perfused with purified water for control.After successful modeling,rats in Group N and M were treated with purified water.Rats in Group A were treated with Atractylenolide I suspension.Group P was given PST suspension by gavage.After treatment,the changes of body weight,intestinal propulsion,fecal water content and histopathological changes of colon were recorded and detected.The content of short chain fatty acids in rat intestine was analyzed by GC-MS;The structure and abundance of intestinal flora in rat feces were analyzed by 16 S r RNA method.Results Atractylenolide I increased the fecal water content of rats: after three weeks of CDT intervention,the fecal water content of rats in Groups M,P and A were significantly lower than that in Group N(P<0.01).After three weeks of therapeutic drug intervention,the fecal water content of rats in Groups N,P and A were significantly higher than that in Group M(P<0.01);Atractylenolide I can promote intestinal movement: after three weeks of CDT intervention,the carbon powder propulsion rate of rats in Groups M,P and A were significantly lower than that in Group N(P<0.01).After three weeks of therapeutic drug intervention,compared with Group N,the carbon powder propulsion rate in group M was significantly lower(P<0.01).Compared with Group M,the carbon powder propulsion rate of rats in Groups P and A were significantly higher than that in the Group M(P<0.01),suggesting that after the intervention of therapeutic drugs,the intestinal motility of STC model rats was restored,and there was no significant difference between the two groups.Atractylenolide I could increase the content of 5-HT in colon of rats: after3 weeks of treatment,the content of 5-HT in colon tissue of rats in M group was significantly decreased compared with that in N group(P<0.01);Compared with M group,5-HT content in P and A group was significantly increased(P<0.01).Effect of Atractylenolide I on intestinal flora of rats: at the taxonomic level,compared with Group N,the abundance of intestinal Firmicutes in Group M increased(P<0.05).After the intervention of therapeutic drugs,compared with Group M,the abundance of Firmicutes in Group P decreased(P<0.05);Compared with Group M,the abundance of Firmicutes in Group A decreased(P<0.05).At the genus classification level,compared with Group M,the abundance of Collinsella in Group P increased(P<0.05);In group A,the abundance of Prevotellaceae＿UCG-003(P<0.05),Bacteroides(P<0.05),Parabacteroides(P<0.05)was increased.Effect of Atractylenolide I on the content of short chain fatty acids in intestinal contents of rats: compared with Group N,the content of acetic acid in intestinal contents of rats in Group M decreased significantly(P<0.01);After the intervention of therapeutic drugs,the content of acetic acid in Group P and A were significantly higher than that in Group M(P<0.01).Compared with Group N,the content of propionic acid in intestinal contents of Group M rats decreased significantly(P<0.01);Compared with Group M,the content of propionic acid in intestinal contents of rats in Groups P and A increased significantly(P<0.01).Compared with Group N,the content of butyric acid in intestinal contents of Group M decreased significantly(P<0.01);M.There was no significant difference in butyric acid content between Groups P and A.Correlation analysis between short chain fatty acids and intestinal flora: acetic acid has a significant positive correlation with Collinsella,Monoglobus,Bacteroides,Treponema,etc.Propionic acid and Candidatus＿Saccharimonas,Bacteroides and Parabacteroides showed positive correlation.Butyric acid and norank＿f＿＿Oscillospiraceae 、 Candidatus＿Saccharimonas showed positive correlation;It is negatively correlated with Psychrobacter.Conclusion Atractylenolide I can promote intestinal peristalsis,increase fecal water content and alleviate constipation symptoms in rats.Atractylenolide I can positively regulate the diversity of intestinal flora in rats.The mechanism may be to improve the constipation state of rats by increasing the proportion of Bacteroides and Parabacteroides,and then promoting the release of propionic acid. |
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