The Expression And Clinical Significance Of NSMCE2 In Breast Cancer

Objective: To analyze the expression of NSMCE2 in breast cancer,study the relationship between the expression level of NSMCE2 in breast cancer patients and clinical pathological features,and evaluate the relationship between the expression level of NSMCE2 and the survival and prognosis of patients,so as to provide new biomarkers for the diagnosis and prognosis of breast cancer.Methods: Firstly,analysis of TCGA database:(1)Analyze whether the expression of NSMCE2 in different between the breast cancer and normal breast issues,and evaluate the diagnostic value of NSMCE2 molecule by using the receiver operator characteristic curve.(2)To evaluate the correlation between the expression of NSMCE2 and clinicopathological features.(3)Use the survival package in R software to carry out Kaplan-Meier analysis,and compare the difference of P value between the group survival by bilateral Log-rank test.Then,univariate regression and multivariate regression Cox analysand were used to evaluate the prognostic value of NSMCE2.Secondly,the paraffin samples of breast cancer and benign breast tumors,clinical information and follow-up information of breast cancer patients were collected.Then,Immunohistochemistry was used for detection:(1)The expression of NSMCE2 in benign and malignant breast tumors was detected by immunohistochemistry.(2)The relationship between the expression level of NSMCE2 and clinicopathological features was statistically analyzed.Results: The information analysis of TCGA database showed that:(1)The expression of NSMCE2 in breast cancer is significantly higher than that in normal breast tissues.According to the analysis of the receive operator characteristic curve,the area under ROC curve is 0.843(95% confidence interval[CI]=0.822–0.865),and this gene can well distinguish normal breast tissue from breast cancer.(2)The expression of NSMCE2 was correlated with the anatomical location of the primary focus(P=0.02),histological type(P<0.01),the status of progesterone receptor(P=0.033),lymph node metastasis(P=0.017),molecular typing of PAM50(P<0.01),Overall survival(P=0.19),and Diseasespecific survival(P=0.12).According to the results of survival analysis,the high expression level of NSMCE2 is related to the poor OS(P=0.018)and DSS(P=0.009).(3)Cox multivariate analysis showed that tumor stage,age of patients and expression of NSMCE2 were independent prognostic factors of OS and DSS in breast cancer patients.According to IHC analysis:(1)NSMCE2 was expressed in both benign breast tumors and breast cancer.Statistical analysis showed that the expression of NSMCE2 protein in breast cancer samples was different from that in benign breast tissues(P<0.001).(2)The expression of NSMCE2 protein in patients with breast cancer is correlated with the stage of breast cancer(P<0.0462),but not with other pathological features.Conclusions: NSMCE2 is highly expressed in breast cancer,and the subgroup with high expression level has worse overall survival and disease specific survival than the low expression subgroup.It suggests that NSMCE2 is expected to be a new biomarker for the diagnosis and prognosis of breast cancer.