MicroRNA-122 Targets Regulating Panx1 And Inhibits Proliferation,Migration And Invasion Of A549 Cells

[Objective]: Based on bioinformatics,the expression of Pannexin1(Panx1)in human non-small cell lung cancer(NSCLC)tissues was detected,the effects of micro RNA-122(mi R-122)on the expression of the target gene Panx1,and the ability of cell proliferation,migration and invasion in human NSCLC A549 cells were investigated.[Methods]: Using bioinformatics database,the expression of Panx1 in lung cancer tissues and its relationship with mi R-122 were collected and verified.Human lung cancer cell line A549 was selected and transfected with mi R-122 mimics to overexpress in the cells.Detection of m RNA expression levels of mi R-122 and Panx1 in human lung cancer A549 cells by quantitative real-time fluorescence PCR(q RT-PCR);CCK-8 assay was used for cell proliferation;Clone formation assay was used for clone ability of cells;Flow cytometry was used for apoptosis;Transwell assay was used for ability of cell migration and invasion.[Results]: TCGA database and HPA database analysis showed that compared with normal lung tissues,the expression of Panx1 m RNA(P < 0.001)and protein(P < 0.001)in lung cancer tissues increased significantly.The results of cell experiments show that mi R-122 expression was significantly increased in A549 cells transfected with the mi R-122 mimics compared with the control group and the mi R-122 NC group(P < 0.001),while both Panx1 m RNA and protein expression were significantly down-regulated(P < 0.05);Compared with the NC group,the apoptosis rate of the mi R-122 mimics transfection group increased significantly(P < 0.01),but the ability of cell proliferation,migration and invasion decreased significantly(P < 0.01).[Conclusion]: Compared with normal lung tissues,the expression level of Panx1 in lung cancer tissues was significantly increased,and mi R-122 targeted to regulate Panx1 expression,inhibited cell proliferation,migration and invasion ability,and promoted lung cancer cell apoptosis,and provided new treatment strategies and ideas for the clinical treatment of lung cancer.