| BackgroundAcute myocardial infarction(AMI)is a major cause of mortality worldwide and in the Chinese population.It is generally manifested as acute onset,and easy to attack at night,sometimes in the early onset of clinical symptoms are not typical,often difficult to get early diagnosis and timely treatment,leading to its high mortality and rising trend,has become one of the main diseases threatening human health.Therefore,early diagnosis and intervention is the key to reduce AMI mortality.However,at present,cardiac troponin(CTN)still has some defects as the gold standard for the diagnosis of AMI,and reperfusion therapy to restore coronary blood flow will aggravate the secondary injury of myocardium.Therefore,there is an urgent need to find a new target or a new way to improve the diagnosis and treatment ability of AMI.We collected the clinical diagnosis of AMI patients underwent Percutaneous coronary interention menstruation(Percutaneous coronary intervention,PCI)before and after the plasma micro RNA in secrete body outside,through the building and the second generation sequencing technology,get complete AMI patients plasma outside body whole genome information of micro RNA secretion,and analysis of the key differences between the expression of micro RNA with AMI,reveal whether plasma outside secrete body can be a warning signal,AMI diagnostic or therapeutic targets.In recent years,it has been found that exosomes contain a series of bioactive substances such as nucleic acids,proteins and lipids,and can play a role in information transmission between a variety of cells,and become a new medium for communication between cells.It not only participates in a variety of normal physiological processes of the human body,but also participates in the occurrence and development of many diseases,including tumor,infectious disease,nervous system disease and heart disease.At present,a number of experiments have shown that the type and content of exosome micro RNA(mi RNA)have significantly changed in AMI,which may be involved in the occurrence and disease progression of AMI,and become a potential diagnostic marker or therapeutic target,with certain clinical value.ObjectiveIn this topic,we collected the clinical diagnosis of AMI patients underwent Percutaneous coronary interention menstruation(Percutaneous coronary intervention,PCI)before and after the plasma outside the body of the mi RNA secretion,and through the library building and the second generation sequencing technology,get complete AMI patients plasma secrete micrornas whole genome information,analysis of the key differences between the expression of micrornas with AMI,reveal the plasma outside can secrete body of micrornas as predictors of AMI,diagnostic or therapeutic targets.MethodsWe selected 10 inpatients(8 males and 2 females)who were diagnosed with AMI and met the standards of sodium and discharge in the Department of Cardiovascular Medicine of our hospital from October to December 2019 to participate in the study.First,we extracted plasma exosome mi RNAs from patients before and after PCI surgery,and used nucleic acid analyzer to determine the concentration and fragment analysis of the extracted plasma exosome mi RNAs.Then we constructed a library of the extracted plasma exosome mi RNA,and used nucleic acid analysis instrument to determine the concentration of the constructed library and analyze the fragments.After that,we performed second-generation sequencing on the 9 groups of samples that met the requirements of the computer,and obtained the complete genomic information of the plasma exosome mi RNA.After statistical analysis and bioinformatics analysis,the mi RNAs with significant expression differences before and after PCI surgery were obtained(down-regulated were let-7d,let-7g,mi R-4695,mi R-423-5p,mi R-148,mi R-103,mi R-30,mi R-331,mi R-425,mi R-120;Mi R-199 a,mi R-423-3p,let-7c,mi R-125,mi R-10,mi R-143,mi R-125)were upregulated.The relationship between mi R-199 a and AMI and its potential value in the diagnosis and treatment of AMI were analyzed.In addition,we predicted the target m RNAs of mi RNAs with different expressions,carried out enrichment analysis of mi RNAs,and constructed a GO analysis network to annotate the biological processes,cellular components and molecular functions that mi RNAs may participate in,different mi RNAs were selected for real-time fluorescence quantitative PCR verification to guide subsequent cell-level studies or animal experiments.Results1.The plasma exosome mi RNA library of acute myocardial infarction patients before and after PCI surgery was successfully constructed,and the complete genome-wide information of plasma exosome mi RNA of AMI patients was obtained by second-generation sequencing technology.2.Before and after PCI surgery,the expression levels of plasma exosomes let-7d,let-7g,mi R-4695,mi R-423-5p,mi R-148,mi R-103,mi R-30,mi R-331,mi R-425 and mi R-120 decreased in patients with acute myocardial infarction.The expression levels of mi R-199 a,mi R-423-3p,let-7c,mi R-125,mi R-10,mi R-143,and mi R-125 were increased.3.These exosomal mi RNAs may be involved in biological processes such as signal transduction,cell communication,nucleic acid transfer,molecular phosphorylation,and may also act as cellular components that constitute cytoplasm and receptor complexes.Conclusions1.The mi RNA expressions of various exosomes in plasma of AMI patients were significantly different before and after PCI operation.Therefore,exosomal mi RNAs may be involved in the occurrence and development of acute myocardial infarction and have the potential to be used as diagnostic markers or therapeutic targets for AMI.2.Exosome mi RNAs are involved in the occurrence and development of AMI in multiple stages of the biological process,and have certain potential for Y early warning,diagnosis and treatment of AMI. |
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