Effects Of Tianxingjianyikang Granules On Glucose And Lipid Metabolism In T2DM Rats

Objective: To observe the effects of Tianxing Jianyikang granules on glucose and lipid metabolism and liver oxidative stress in type 2 diabetes(T2DM)rats induced by high-fat diet combined with low-dose streptozotocin(STZ)tail-vein injection.Methods:Sixty SPF male SD rats were selected at the age of 6 weeks.After adaptive feeding for 1 week,the rats were divided into blank group,model group,positive control group,tianxing Jianyikang granules high-dose,medium-dose and low-dose groups according to their body weight,with 10 rats in each group.The blank group was fed with ordinary diet for 4 weeks,and the model group was fed with high fat diet for 4 weeks.4 weeks later,streptozotocin(STZ)was injected into the tail vein to establish the animal model of type 2 diabetes mellitus(T2DM).Except for the blank group,the other 5 groups were injected with STZ solution in standard tail vein at35mg/kg,and the blank group was injected with normal saline at the same volume ratio.Seventy-two hours after streptozotocin(STZ)injection,blood samples were collected from tail vein to measure blood glucose,and three consecutive random blood glucose values ≥ 16.7mmol/L were considered as successful modeling of diabetic rats.Rats whose blood glucose level did not reach the standard were supplemented with streptozotocin 30mg/kg in caudal vein.After successful modeling,drug intervention was carried out.The method of administration was once a day,the time of administration was9:00-11:00 a.m.,and continuous gavage was carried out for 28 days.Among them,blank group and model group were intragastrated with normal saline at the same volume ratio,positive control group was intragastrated with metformin hydrochloride solution according to the standard 0.25g/kg/d,tianxing Jianyikang granule low-dose,medium-dose and high-dose groups were intragastrated with Tianxing Jianyikang granule solution according to the standard 1.35g/kg,2.7g/kg and 5.4g/kg,respectively.Within four weeks,the general state of rats in each group was recorded by observation,including weekly body weight and Fasting Blood Glucose(FBG);Daily food intake,water intake,etc.Oral Glucose Tolerance(OGTT)of rats in each group was measured on the day of animal model sampling.Total Cholesterol(TC),Triglyceride(TG),High Density Lipoprotein(HDL-C),Low Density Lipoprotein(LDL-C),Fasting Insulin(FINS),Aspartate Aminotransferase(AST),Alanine Aminotransferase(ALT),Creatinine(Cre),Urea Nitrogen(BUN)and other serum indexes were detected by blood samples from abdominal aorta.After blood sampling,the liver weight of rats in each group was weighed and the liver body ratio was calculated.Part of liver tissue was collected for HE staining to observe the pathological morphology of liver tissue.Results:1.Glucolipid metabolism and insulin sensitivity: Compared with model group,Tianxing Jianyikang granule low-dose and high-dose groups could significantly alleviate the phenomenon of weight loss,food intake increase and hepatobody weight increase in T2 DM rats in a short period of time(P<0.01).Tianxingjianyikang granules could increase HDL-C level in T2 DM rats(P<0.01).FBG,TC,TG and LDL-C decreased in T2 DM rats(P<0.01).In addition,FINS,IR and AUC were decreased(P<0.01).2.Liver and kidney function and liver pathological morphology:Tianxingjianyikang granules can reduce the levels of AST,ALT,Cre and BUN in serum of T2 DM rats(P<0.01).Tianxingjianyikang granules can improve the balloon-like changes of hepatocytes in T2 DM rats and protect liver tissue.Conclusion:1.Tianxingjianyikang granules can reduce fasting blood glucose,food intake and water intake of T2 DM rats,and slow down the degree of weight loss2.Tianxingjianyikang granules can improve insulin sensitivity of T2 DM rats,effectively enhance glucose tolerance of T2 DM rats,and protect the function of ISlet B cells;The liver weight ratio of T2 DM rats was decreased,the indexes of blood lipid were improved,the deposition of liver lipid was reduced,and the glucose and lipid metabolism were improved.3.Tianxingjianyikang granules can effectively regulate related indexes of liver and kidney function in T2 DM state,and have a certain protective effect on liver and kidney function.