Preliminary Study On The Effect And Mechanism Of FOXP1 On The Malignant Biological Behavior Of Ovarian Cancer Cells

Objective:In this study,we propose to explore the effect of FOXP1 on malignant biological processes such as proliferation,apoptosis,migration,invasion,angiogenesis and EMT of ovarian cancer cells and its regulatory mechanism in the p38-MAPK signaling pathway by changing the expression level of FOXP1 in ovarian cancer cells at the in vivo and in vitro levels,and to initially clarify how FOXP1 plays a role in the malignant biological process of ovarian cancer cells.To lay a certain experimental foundation for finding new ovarian cancer diagnosis and treatment targets.Methods:FOXP1 was used as the center of the study,immunohistochemistry(IHC)and Western blot were used to detect the expression of FOXP1 in clinical samples of human ovarian cancer and different ovarian cancer cell lines,and the prognostic correlation between FOXP1 and ovarian cancer was evaluated.Ovarian cancer cells were transfected using small interfering RNA techniques to specifically silence FOXP1 and verify their effectiveness by real-time quantitative PCR(RT-q PCR)and Western blot.The effects of silent FOXP1 on the ability of ovarian cancer cells to proliferate,migrate,invade,apoptosis,EMT and angiogenesis were observed using cell proliferation assay,colony formation assay,transwell and scratch assays,flow cytometry,Western blot,Matrigel tube formation assay and other phenotypic experiments,and through Western blot and corresponding phenotypic experiments detected changes in associated proteins in the p38-MAPK signaling pathway after foxp1 silencing and changes in the malignant biological characteristics of corresponding ovarian cancer cells.Finally,the changes in the growth capacity of ovarian cancer cell lines after FOXP1 silencing in xenograft mice model in vivo were investigated by subcutaneous injection of ovarian cancer cells after silencing FOXP1.Results:1.FOXP1 level was increased in ovarian cancer tissues and elevated expression of FOXP1 have reduced probability of survival.2.Silencing FOXP1 can significantly inhibit the proliferation,migration,invasion,EMT and angiogenesis process of ovarian cancer cells,and promote the apoptosis of ovarian cancer cells.3.Silencing FOXP1 can significantly inhibit the biological processes of proliferation,migration,invasion,EMT and angiogenesis of ovarian cancer cells by inhibiting the p38-MAPK signaling pathway,and inhibiting the p38-MAPK signaling pathway promotes apoptosis.4.Silencing FOXP1 can inhibit the growth of ovarian cancer cells in vivo and reduce angiogenesis at the tumor site.Conclusion:This study confirmed that the expression of FOXP1 was elevated and associated with poor prognosis in patients with ovarian cancer,and it was clear that the proliferation,apoptosis,migration,invasion,EMT and angiogenesis of ovarian cancer cells in vitro were inhibited by negative regulation of the p38-MAPK signaling pathway after silencing FOXP1,and tumor growth and angiogenesis were inhibited in vivo.In summary,the results of this study will help to understand the malignant biological effect of FOXP1 in ovarian cancer cells and its mechanism of action,and hope that it will provide a new experimental basis for the development of drugs targeted to treat ovarian cancer,and hope that it can be applied to the clinical diagnosis and treatment of ovarian cancer as soon as possible.