PDZ-binding Kinase In Expression And Clinical Significance Of Glioblastoma

Objective:The core genes closely related to the occurrence and development of Glioblastoma(GBM)were screened out by bioinformatics analysis,and the expression and clinical significance of the core genes in GBM were discussed,so as to find new targets for gene therapy of GBM.Methods:Differentially expressed genes in non-tumor brain tissue and GBM tissue were screened by bioinformatics tool GEO database.GO and KEGG pathway analysis was performed on these differentially expressed genes through the DAVID database to study their gene functions.Using Cytoscape software in STRING database to obtain core genes significantly associated with GBM.Using UALCAN online survival analysis tool and multivariate Cox regression analysis model,the core gene and independent risk factor associated with poor prognosis of GBM were identified as PDZ-binding kinase(PBK).Subsequently,the TIMER database was used to study the relationship between the expression of PBK and tumor immune infiltrating cells in the GBM dataset.Finally,the expression of PBK was double verified by immunohistochemical detection of GEPIA,TCGA,CGGA databases and clinical GBM specimens.Results:227 differentially expressed genes significantly associated with GBM were screened by bioinformatics.GO and KEGG analysis showed that it was involved in a variety of biological behaviors and signaling pathways.The PPI interaction network analysis map screened out 10 core genes related to GBM.The results of survival analysis showed that the core gene was PBK gene,which was associated with poor prognosis of GBM patients(P<0.05)and was an independent risk factor for poor prognosis(P=0.019).The immune infiltration analysis of the TIMER database showed that PBK was significantly positively correlated with CD8~+T cells(Rho=0.256,P=2.51e-03),and positively correlated with CD4~+T cells(Rho=-0.349,P=2.91e-05),Neutrophils(Rho=-0.278,P=1.02e-03)and dendritic cells(Rho=-0.225,P=8.19e-03)were significantly negatively correlated.Finally,the immunohistochemical analysis of GEPIA,TCGA,CGGA database and GBM specimen data showed that the expression level of PBK in human brain GBM tissue was significantly higher than in non-tumor brain tissue(P<0.05).Conclusion:PBK is a core gene in the progression of GBM,and its high expression is associated with poor prognosis of GBM patients,and may be related to the cell cycle and immune microenvironment of GBM.It is suggested that PBK can be used as a biomarker for the diagnosis and prognosis of GBM.