Neural Stem Cells Are Stimulated To Differentiate Into Neurons Using Electrical Currents Generated By A Self-driven Electromagnetic Generator

Objective:Cerebral hemorrhage traumatic brain injury Degenerative diseases of the central nervous system all lead to neuronal death,resulting in decreased motor sensory cognition and even loss.Neural stem cells(NSCs)exist in the central system,and NSC is a kind of self-renewing stem cells.Experiments have proved that NSCs transplantation has a positive therapeutic effect on ischemic stroke,traumatic brain injury,neurodegenerative diseases and many other central nervous system diseases.Studies have shown that local surface charges generated by electrical stimulation can promote the growth of nerve fibers and induce nerve cells to secrete various growth factors including neurotrophic factors.In addition,electrical stimulation can regulate the differentiation of neural stem cells into neurons.However,most stimulation devices require an external power source or repeated periodic charging,and require the conversion of a large current or voltage to a known operating current or voltage suitable for body stimulation before further application,which limits their range of application.The aim of this study was to use a self-actuated electrical stimulation device to directly stimulate the nerve stem to promote the differentiation and maturation of stem cells.Methods:Separation and cultivation of neural stem cells to extend every 7 days,use 2-3 generation of neural stem cells,neural stem cells between vaccination in 6 orifice,the second day after inoculation will drive the generator to produce electricity by special cells electrical stimulator to the orifice of electrical stimulation,in 7 days after inoculation,which stimulates cells for five days to charge the sample,application CCK8,q PCR and Western Blot Immunofluorescence was used to detect the genes and proteins symbolically expressed in active neurons.Later,Image J was used to analyze the proportion of neurons and the length of their protrusions.Results:After 5 days of stimulation at an output current of 2 m A from the self-driven electrical stimulation generator,the neural stem cell differentiation-related genes MAP2,TUJ1 neuronal signature protein,and the proportion of neurons were significantly increased,while the astrocyte differentiation-related genes GFAP,astrocyte signature protein,and the proportion of astrocytes were significantly decreased.Conclusion:The results show that the electrical energy output by the self-driven electromagnetic generator can promote the differentiation of neural stem cells into neurons and astrocytes,and can improve the length of differentiated neurons.The increase in the number and length of neuronal processes can promote the connection between neurons to form neural networks,and further promote the repair of the nervous system.These results provide a theoretical basis for the application of self-driven generator in neurotraumatic diseases and neurodegenerative diseases.