Efficacy Of Intravenous Administration And Tumor Administration-mediated 5-ALA Photodynamic Therapy On Nude Mouse Ovarian Cancer Graft Tumors

Objective:Photodynamic therapy(PDT)is a promising tumor treatment method.5-aminoketovaleric acid(5-ALA)is a commonly used drug,and the main advantages of5-ALA includes: small side effects,good therapeutic effect and so on.At present,the therapeutic effect of 5-ALA on animal ovarian cancer transplant tumors has not been fully studied.In this study,the effect of 5-ALA based PDT on subcutaneous transplant ovarian cancer was carried out in nude mice,and we also studied whether different modes of administration(tail vein/tumor)produced differences in efficacy.Methods:48 female Balb/c nude mice aged 4-6 weeks were randomly divided into control group,simple intravenous administration group,simple local administration group,simple laser group,intravenous administration + laser group and local administration + laser group after successful modeling.Mice in the treatment group were treated once every three days for a total of 5 treatments,and no treatment was given after modeling in the control group.During treatment,the size change of subcutaneous graft tumors in mice was measured,mice were sacrificed after treatment,tumor tissues were removed,tumor inhibition rates were compared,HE staining was performed to observe the morphological changes of tumor cells,and changes in serum tumor markers CA125 and HE4 were compared.Results:Compared with the control group,the tumor volume of group E and group F decreased significantly(p<0.05).The tumor volume in group E was larger than that in group F(p<0.05),and the tumor inhibition rate in group E was slightly lower than that in group F(44.84% vs 47.27%)(p<0.05).Obvious necrosis of tumor cells in PDT group was observed by HE staining.The levels of serum CA125 and HE4 in group E and group F were significantly lower than those in the control group(p<0.05),but there was no significant difference between group E and group F.Compared with the control group,there was no significant difference in each index between the simple laser group and the simple administration group.Conclusion:5-ALA-PDT is effective in the treatment of ovarian cancer in nude mice,and the efficacy of tumor administration and intravenous administration cannot be considered to be significantly different,and neither administration alone nor light alone can produce therapeutic effects.More research is needed in the future to explore the therapeutic role of 5-ALA-PDT in ovarian cancer.