The Mechanism Of FKBP8 Inhibiting Apoptosis Of Thyroid Cancer Cells

Objective:Thyroid malignancy(TC)is a relatively common malignancy in the neck,and understanding the underlying molecular mechanisms of TC will help to develop novel targeted therapy and improve the survival rate of patients with refractory thyroid cancer.FKBP8 is an important regulator involved in the process of apoptosis.Studies have shown that in lung and breast cancer,reducing FKBP8 can promote the development of this tumor to a certain extent.However,the role of FKBP8 in TC and the mechanism of its involvement in programmed cell death remain unclear.Therefore,this paper will explore the correlation between the expression level of FKBP8 and the prognosis of thyroid cancer patients;study the effect of FKBP8 on the proliferation and apoptosis of thyroid cancer cells;and preliminarily explore the molecular mechanism of FKBP8 regulating the apoptosis sensitivity of thyroid cancer cells.Methods:The TCGA database was used to analyze the expression levels of FKBP8 and GPX4 in thyroid cancer patients.At the same time,immunohistochemical techniques were used to further verify the expression levels of FKBP8 and GPX4 in thyroid cancer patients,and their correlation with the pathological characteristics of patients was analyzed.RNA interference technology was used.si-FKBP8 and si-GPX4 were transfected into 8305 C and B-CPAP cells,respectively,to construct cell lines with low expression of FKBP8 and GPX4.The thyroid cancer transiently transfected cell lines were identified by q RT-PCR and Western Blot techniques;CCK-8 The effect of FKBP8 and GPX4 expression changes on the proliferation ability of thyroid cancer cells was detected by experiment and Edu experiment respectively;the apoptosis of thyroid cancer cells with altered FKBP8 and GPX4 expression in adherent culture was detected by flow cytometry.The correlation between FKBP8 and HSPB1 at the mRNA level was explored.To preliminarily explore the molecular mechanism of the effect of FKBP8 expression on the sensitivity of thyroid cancer cells to apoptosis.Results:In the TCGA database,it was found that the expression levels of FKBP8,GPX4 and HSPB1 were all elevated in the tumor tissue of thyroid cancer patients,and the expressions of FKBP8,GPX4 and HSPB1 were all significantly positively correlated.Immunohistochemical results showed that the expressions of FKBP8 and GPX4 were significantly increased in thyroid cancer tissues,and the high expression levels of both were significantly correlated with poor prognostic factors in thyroid cancer.The later the clinical stage of tumor tissue,the higher the expression of FKBP8 and GPX4.Knockdown of the expression of FKBP8 and GPX4,respectively,can significantly reduce the proliferation level of thyroid cancer cells,and further found that the apoptosis rate of the two cells was significantly increased.Conclusion:Our results suggest that FKBP8 and GPX4 may promote the proliferation of thyroid cancer cells by inhibiting the apoptosis of thyroid cancer cells.This study has certain clinical value in the biological targets of TC therapy and prognosis evaluation.The interaction of FKBP8 with GPX4 and HSPB1 in thyroid cancer cells requires further study.