Research On Changes In Hippocampal 5-HT2A Receptor Number And Depression After Mice Spinal Cord Injury

Objective:This study investigated the correlation between changes in the number of5-Hydroxytryptamine-2A(5-HT2A)receptors and depression after spinal cord injury in mice to provide a reference for studying the formation mechanism,clinical treatment,and prognosis of depression after spinal cord injury.Methods:In the preliminary experiment,the results of the sucrose preference test indicated that compared with the sham operation group,the preference for sucrose water in mice with spinal cord injury was significantly reduced(F(5,60)= 7.029,P<0.0001).The degree of depression was most significant at week 5(t=6.142,df=12,P<0.0001).The SCI group’s 5-HT2 A receptor and PLC protein expression levels were higher than those in the sham operation group(P<0.0001).Experiments were performed at different times determined by preliminary experiments.The open field test,the forced swim test,and the tail-suspension test indicated that the mice with SCI exhibited significant depressive states five weeks after the operation(P<0.0001).The corresponding behavioral indicators of mice intervened with the selective 5-HT2 A receptor inhibitor Ketanserin were significantly improved compared with those in the operation group.Western blot analysis showed that the protein expressions of5-HT2 AR and PLC in the SCI group were significantly increased(P<0.0001).The protein expression after Ketanserin intervention was down-regulated compared to that in the injury group(P<0.0001).Conclusions:An increase in the number of 5-HT2 A receptors in the hippocampal region of mice may be one of the mechanisms leading to the decline in cognitive function and the emergence of depression-like behaviors after spinal cord injury.