Transcription Factor SOX2 Up-regulates DEK To Promote The Progression Of Lung Squamous Cell Carcinoma

Objective: Lung squamous cell carcinoma(LSCC)is a prevalent and progressive subtype of lung cancers.This study was aimed to substantiate the regulatory effect of the SOX2/DEK axis on LSCC development.Methods: Four LSCC cell lines(LTEP-S,SK-MES-1,NCI-H2170,NCI-H520)and one normal lung epithelial cell line BEAS-2B for experiments,and LSCC tissues(n=83)and adjacent normal tissues for SOX2 expression by q RT-PCR and Westernblot.Kaplan-Meier was used to analyze the correlation between LSCC-SOX2 expression and patient outcome.The CCK-8 assay was used to examine the inhibitory effect of silencing SOX2 on the viability of LSCC cells.SOX2 binding to DEK was determined by applying Ch IP.Loss-of-function and loss of function analysis were then performed on LSCC cells,applying CCK-8 and Transwell assays to examine cell viability,migration,and invasive potential.Results: SOX2 and DEK were upregulated in LSCC tissues and cells.SOX2 overexpression was positively correlated with poor prognosis in LSCC patients.Silencing of SOX2 impaired the viability,migration,and invasive potential of LSCC cells.Moreover,SOX2 activated DEK expression,DEK expression in LSCC tissues was positively correlated with SOX2 expression,and silencing DEK attenuated the malignant behavior of LSCC cells.Conclusion: 1.SOX2 is involved in the formation of LSCC and is highly expressed in LSCC tissues,resulting in a poor prognosis of LSCC.2.Silencing of the SOX2 gene inhibited the proliferation,migration,and invasive potential of LSCC cells.3.SOX2 activates the downstream expression of DEK genes and drives the malignant behavior and tumor growth of LSCC cells.4.Silencing of the DEK gene significantly inhibited the viability,migration and invasion ability of LSCC cells,and inhibited the malignant phenotype of LSCC cells.