Data Mining Of Renal Fibrosis And Preliminary Exploration Of Key Candidate Molecules For Regulating Fibrosis

Objective:1.In this study,we analyzed the renal tissue microarray data of kidney fibrosis induced by folic acid from the NCBI GEO database and described the main events in the development of kidney fibrosis and their sequence of development.2.Searching and identifying candidate molecules that play a key regulatory role in the development of renal fibrosis Methods:The database downloaded from NCBI GEO was processed and analyzed using the R package GEO quary(Davis and Meltzer,2007),including principal component analysis,hierarchical cluster analysis,and gene set enrichment analysis to describe the main events in the development of renal fibrosis;gene expression and differential analysis was performed for preliminary screening of differential genes,and a rat renal fibrosis model was established to determine the expression levels of the initially screened differential genes at the transcriptional and translational levels.Results:1.In this study,hierarchical cluster analysis and principal component analysis of the GSE65267 database were performed by R package,and the results showed that in this database,the process of renal fibrosis development from the third day,the most significant changes in gene transcript levels within the kidney tissue;GSEA of the same database showed that inflammation,cell growth repair,and fibrosis-related pathways were sequentially highly expressed in the kidney tissue after FA application in the experimental group;KEGG,GSVA,and GO pathway enrichment analysis showed that the seventh to fourteenth day after FA application was the key regulatory stage for fibrotic changes in the kidney.2.Based on the results of differential gene expression analysis in the database and after reviewing the relevant literature,a series of differentially expressed genes were screened out.The results showed that the expression of Cxcl2,Egr2 and Tmem70 were significantly altered in renal fibrosis tissues.Conclusion:During the progression of renal fibrosis related events such as disruption of energy metabolism,inflammation,cellular repair,and fibrosis are sequentially altered,we concluded after preliminary screening that three genes,Cxcl2,Egr2,and Tmem70,could be key candidate molecules for regulation in the development of renal fibrosis.