| Objective In this study,the rats were stimulated with “BL13” point on both sides of chronic obstructive pulmonary disease(COPD)model to observe the changes of pulmonary ventilation function,trachea,lung histopathology,inflammation and oxidative stress,and to investigate the effects of pressing needle on COPD rats.to provide a certain experimental basis for the clinical application of pressing needle in the treatment of COPD.Methods1.Grouping and modeling: 28 SD rats were randomly divided into two groups using random number table,12 rats in group A and 16 rats in group B.Group B was prepared by lipopolysaccharides(LPS)intratracheal drip + fumigation for 10 weeks,and 2 rats were taken at the 4th and 7th weeks of modeling,and the chest cavity was opened to observe the modeling situation.At the eighth week,group B was randomly divided into 6 rats in the model group and6 rats in the pressing needle group,while group A was divided into 6 rats in the blank group and 6 rats in the normal group.2.Intervention: pressing needle group started from the 8th W.Before the intervention,the hair was shaved off at both sides of the “BL13” point,the pressing needle was pierced into the“BL13” point,and the “BL13” point was pressed and rubbed for 2min,every 30 min,for a total of 4 times,the total time of pressing and rubbing was 8 min/d,the rest of the time the pressing needle was fixed with adhesive tape,the intervention was 6 d/W,and the intervention was continuous for 3W.The normal group and model group had the same degree of grasping,fixation and hair removal as the pressing needle group,without other treatments;the blank group did not have hair removal,and the rest was the same as the normal group.3.Index observation: the general condition of rats was observed during the experiment,and after the intervention,the lung function was detected by RM6240 B multi-channel physiological recorder,and the histopathological changes of trachea and lung tissue after HE staining were observed under optical microscope,and IL-10,IFN-γ,SOD and MDA in serum and lung tissue were detected by Elisa method;finally,the data were analyzed by SPSS 22.0 statistical analysis.Results1.General condition: Before intervention: Group A rats had bright white and fine fur,normal diet,good activity,responsive,even respiration and normal diarrhea;compared with Group A rats,Group B rats had yellowish dull and rough fur,decreased food intake,wasted,weakened activity,more unresponsive and occasionally heard panting.After the intervention: compared with the blank group,the rats in the normal group showed no significant changes in diet,diarrhea,mobility and respiration;compared with the normal group,the rats in the model group showed slower hair growth at the shaved area,yellowish,dull and rough hair,decreased food intake,decreased body mass,reduced mobility,and occasionally heard coughing,coughing and wheezing;Compared with the model group,the rats in the pressing needle group had faster hair growth,improved yellow hair color,increased diet,increased body mass,increased activity,and improved cough,sputum and wheezing.2.Lung ventilation function indicators: Compared with the blank group,rats in the normal group had slightly higher ventilation per minute,increased pulmonary elastic resistance(P<0.05)and decreased pulmonary compliance(P<0.05);compared with the normal group,the rats in the model group had significantly lower ventilation per minute,significantly lower pulmonary elastic resistance and significantly higher pulmonary compliance,and the differences were all statistically significant(P<0.01);compared with the model group,the rats in the pressing needle group had significantly lower ventilation per minute,significantly lower pulmonary elastic resistance and significantly higher pulmonary compliance,and the differences were all statistically significant(P<0.01).3.Pathology of trachea and lung tissue: blank group: the epithelium of tracheal mucosa of rats was intact and no inflammatory cell infiltration was seen;compared with the blank group,the epithelium of tracheal mucosa of rats in the normal group was intact(smooth surface),with more ciliated cells and no abnormal cilia on the free surface of ciliated cells;compared with the normal group,the epithelium of tracheal mucosa of rats in the model group was thickened(hairy surface),with more ciliated cells and longer cilia length;Compared with the model group,the epithelial cells in the pressing needle group did not thicken significantly(the surface was smoother),the ciliated cells decreased,and the length of cilia became shorter.Compared with the blank group,the alveolar structure of the rats in the normal group was intact,and very few inflammatory cells were found in the interstitium;compared with the normal group,the alveolar cavity of the rats in the model group was significantly dilated,and the alveolar septum was ruptured and fused to form pulmonary blisters,and inflammatory cells were found in the interstitium;Compared with the model group,the number of fused pulmonary blisters in the lungs of the rats in the pressing needle group was reduced,and the inflammatory cell infiltration in the interstitium was decreased.4.Inflammatory factor indicators: compared with the blank group,the serum IL-10 and IFN-γcontent of rats in the normal group were slightly lower;the lung tissue IL-10 content was slightly higher and IFN-γ content was slightly lower.Compared with the normal group,the serum and lung tissue IL-10 levels in the model group were significantly reduced and IFN-γlevels were significantly increased,and the differences were statistically significant(P<0.01);compared with the model group,the serum IL-10 levels in the pressing needle group were significantly increased and IFN-γ levels were significantly reduced,and the differences were statistically significant(P<0.01).There was a tendency for the IL-10 content in lung tissue to increase,but it was not statistically significant,and the IFN-γ content was significantly reduced,and the difference was statistically significant(P<0.01).5.Indicators of oxidative stress: compared with the blank group,the SOD content of serum and lung tissues of normal rats was slightly higher,the MDA content of serum was not significantly different,and the MDA of lung tissues was slightly lower;compared with the normal group,the serum SOD contents of rats in the model group showed a decreasing trend,and the MDA contents were significantly higher(P<0.01);the lung tissue SOD contents were significantly reduced and MDA contents were increased,and the differences were statistically significant(P<0.01,P<0.05);compared with the model group,the serum SOD content in the pressing needle group was slightly increased,the serum MDA content was significantly decreased(P<0.01),the SOD content in the lung tissue was significantly increased(P<0.01),and the MDA content did not change.Conclusions1.Pressing needle therapy can significantly improve the general condition,pulmonary ventilation function,maintain the integrity of tracheal mucosa,improve emphysema lesions in rats,and delay the development of COPD to a certain extent.2.Pressing needle therapy can increase the content of IL-10 and SOD in serum and lung tissue,reduce the content of IFN-γ and MDA,play an important role in anti-inflammation and antioxidation,and delay the development of COPD to a certain extent. |
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