| Objective Using Iinfrared Thermal Iimage,Microcirculatory Blood Perfusion Units(MBPU)and Gastrointestinal Electrogram,to explore the changes of the body surface temperature of the stomach meridian,MBPU and the effect of moxibustion in healthy volunteers and volunteers with epigastric pain of spleen and stomach deficiency-cold type at different times,and provide a scientific basis for the clinical application of the Midnight-noon Ebb-flow Hour-prescription Method.MethodsThe experiment recruited healthy volunteers and volunteers with epigastric pain of spleen and stomach deficiency-cold type.Observations were carried out at four different hours: Chenshi(7-9a.m.),Shishi(9-11 a.m.),Wushi(11-13 p.m.),and Xushi(19-21 p.m.).The experiment was observed for one hour,and three days later,the next hour was observed.Infrared thermal imager was used to observe and record the body surface temperature of the stomach meridian in the natural state,and analyze and compare the variation law of the body surface temperature of the stomach meridian in the two groups of volunteers at four hours.The characteristics of the stomach meridian MBPU were observed by laser speckle blood flow imaging instrument,and compared with the temperature changes of the stomach meridian;Eight-lead EGEG-8D electrogastrograph was used to record the characteristics of gastrointestinal electrogram in natural and moxibustion state in healthy volunteers and epigastric pain volunteers,including gastric body(lead 1),antrum(lead 2),lesser gastric curvature(lead 3),greater gastric curvature(lead 4),ascending colon(lead 5),transverse colon(lead 6),descending colon(lead 7),rectum(lead 8),and analyzed the changes of gastrointestinal electrogram in different states.Results1.Research results of body surface temperature of stomach meridian based on infrared thermal imaging technology(1)There was no significant difference in the body surface temperature of the stomach meridian between the healthy volunteers at different times.(2)There was no significant difference in the body surface temperature of the stomach meridian between volunteers with epigastric pain at different hours.(3)The body surface temperature of the stomach meridian of the volunteers with epigastric pain was lower than that of the healthy volunteers at Chenshi,Sishi,Wushi,and Shushi,and the difference was the most obvious at Chenshi,and the difference was statistically significant(P<0.05),and other time differences were not statistically significant.2.Research results of gastric meridian MBPU based on laser speckle imaging technology(1)There was no significant difference in gastric meridian MBPU between the healthy volunteers at different times.(2)There was no significant difference in the gastric meridian MBPU between volunteers with epigastric pain at different times.(3)The MBPU of the stomach meridian of the volunteers with epigastric pain was lower than that of the healthy volunteers at Chenshi,Sishi,Wushi,and Shushi,and the difference was the most obvious at Chenshi,and the difference was statistically significant(P<0.05),and other time differences were not statistically significant.3.Results of Gastrointestinal Electrogram Study(1)Changes of gastrointestinal electrogram before and after moxibustion in the same group of volunteers at different times1)Changes of gastrointestinal electrogram before and after moxibustion in healthy volunteers at different times(1)There was no difference in the mean amplitude of the gastrointestinal electrogram in healthy volunteers before moxibustion at different times;there was no difference in the mean amplitude of the gastrointestinal electrogram in healthy volunteers after moxibustion at different times.(2)After moxibustion,compared with the healthy volunteers before moxibustion,most of the mean amplitudes of gastrointestinal electrograms did not change significantly at Chenshi,Sishi,Wushi,and Shushi,and only some leads showed an increase in the mean amplitude(P<0.05).2)Changes of gastrointestinal electrogram before and after moxibustion in volunteers with epigastric pain at different times(1)There was no difference in the mean amplitude of the gastrointestinal electrogram in volunteers with epigastric pain at different times;there was no difference in the mean amplitude of the gastrointestinal electrogram after moxibustion in volunteers with epigastric pain at different times.(2)After moxibustion,the mean amplitudes of gastrointestinal electrograms increased in the volunteers with epigastric pain compared with those before the moxibustion themselves(P<0.05),at Chenshi,Sishi,Wushi,and Shushi.(2)Differences of gastrointestinal electrograms before and after moxibustion in the two groups of volunteers at the same time1)At Chenshi,there was a difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers before moxibustion,and the difference was statistically significant(P<0.05).After moxibustion,there was no significant difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers.2)At Sishi,there was a difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers before moxibustion,and the difference was statistically significant(P<0.05).After moxibustion,there was no significant difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers.3)At Wushi,the mean amplitudes of gastrointestinal electrical leads of the volunteers with epigastric pain before moxibustion were lower than those of the healthy volunteers,but only the transverse colon leads were significantly different between the two groups(P<0.05).After moxibustion,there was no significant difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers.4)At Xushi,there was a difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers before moxibustion,and the difference was statistically significant(P<0.05).After moxibustion,there was no significant difference in the mean amplitude of gastrointestinal electrogram between the two groups of volunteers.Conclusions1.The body surface temperature and the MBPU of the stomach meridian of the volunteers with epigastric pain were lower than that of the healthy volunteers,and they were most obvious at Chenshi,when the meridian belongs,the mapping of the meridian to the disease state of the corresponding viscera is more obvious.2.The gastrointestinal function of the volunteers with epigastric pain was weaker than that of the healthy volunteers,most obvious at Chenshi,the disease state of the viscera was more obvious when the meridians belonged.3.Moxibustion at Fenglong acupoint on the stomach meridian can improve the gastrointestinal function of volunteers with epigastric pain of spleen and stomach deficiency-cold type,moxibustion at Fenglong point has a certain therapeutic effect on epigastric pain of spleen and stomach deficiency-cold type.4.The moxibustion effect of Fenglong acupoint is more obvious at Chenshi and Sishi than at Wushi and Xushi,the moxibustion effect of acupoints is more obvious when the meridians belong,and if acupoints are selected along the meridians on time,other acupoints(not Wushu points)on the meridians can be selected for treatment. |
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