| Objective: This study intends to explore Augmenter of liver regeneration in Diabetic kidney disease(DN)mouse model and Diabetic kidney disease patients.ALR,Oxisome proliferator-activated receptor alpha(PPARα),Carnitine palmitoyltransferase(Carnitine palmitoyltransferase)1,CPT1),which laid a foundation for the subsequent exploration of the correlation between ALR,PPARα and CPT1 and whether regulating ALR level might change lipid synthesis and fatty acid metabolism in renal tubular cells,and provided a new direction for the treatment of DN.Methods:1.DN mice: The adult male C57 mice were divided into blank group,control group and experimental group.The control group was selected with 0.1M sodium citrate buffer,and injected intraperitoneally at the same dosage as the experimental group for five days.The experimental group was modeled.Streptozotocin(STZ)was injected intraperitoneally at 80 mg/kg for 5 days.Blood and urine samples were collected before the mice were sacrificed,and blood glucose levels,urea,creatinine,cystatin C,and 24-hour urine protein were measured.Kidney tissue paraffin section for pathological examination.The expression levels of ALR,PPARα and CPT1 m RNA were detected by reverse transcription-polymerase chain reaction(RT-PCR).The expression levels of ALR,PPARα and CPT1 were detected by immunohistochemistry(IHC)and western blot(Western-blot,WB).2.DN patients: The database specimens of inpatients who underwent renal puncture in the Nephrology Department of the Affiliated Hospital of Zunyi Medical University in the past 5 years were collected.The protein expression levels of ALR,PPARα and CPT1 in the two groups were detected by IHC.Results:DN mice: 1.Determination of biochemical indicators: blood sugar: the experimental group was significantly higher than the control group and the blank group(P<0.01),and there was no significant difference between the control group and the blank group(P>0.05);urea: Compared with the control group and the blank group,the experimental group was significantly higher(P<0.01),and there was no significant difference between the control group and the blank group(P>0.05).Significantly increased(P<0.01),there was no significant difference between the control group and the blank group(P>0.05);creatinine: the experimental group was significantly higher than the control group and the blank group(P<0.01),the control group and the blank group were significantly higher(P<0.01).There was no significant difference between the groups(P>0.05);cystatin C: the experimental group was significantly higher than the control group and blank group(P<0.01),and there was no significant difference between the control group and blank group(P>0.05).2.Kidney pathology: Compared with the control group and the blank group,the experimental group showed glomerular hypertrophy,increased mesangial matrix,and increased number of cells in the renal cortex.3.Immunohistochemistry: In the kidney tissue of mice in the experimental group,the expression of ALR protein was higher than that of the control group and the blank group(P<0.05),and the protein expressions of CPT1 and PPARα were lower than those of the control group and the blank group(P<0.05).There was no significant difference between the group and the blank group(P>0.05).4.Western blotting: In the kidney tissue of mice in the experimental group,the expression of ALR protein was higher than that of the control group and the blank group(P<0.05),and the protein expressions of CPT1 and PPARα were lower than those of the control group and the blank group(P<0.05).There was no significant difference between the group and the blank group(P>0.05).5.Reverse transcription polymerase chain reaction: In the kidney tissue of mice in the experimental group,the expression of ALR m RNA was higher than that of the control group and blank group(P<0.05),and the m RNA expression of CPT1 and PPARα was lower than that of the control group and blank group(P<0.05),there was no significant difference between the control group and the blank group(P>0.05).DN patients: 1.Kidney pathology: compared with the control group,the cortical tissue of the kidneys in the observation group showed that some glomeruli were significantly enlarged,and there were obvious sclerotic changes,the basement membrane was significantly thickened,and the mesangial matrix was relatively increased.There were fine particles and vacuolar degeneration in tubules,and some tubulointerstitial hyperplasia.2.Immunohistochemistry: The expression of ALR protein in the kidney tissue of the observation group was higher than that of the control group(P<0.05).The protein expressions of CPT1 and PPARα were lower than those in the control group(P<0.05).Conclusion: 1.The expression levels of ALR m RNA and protein in the kidney tissue of DN mice were significantly increased,while the m RNA and protein expressions of CPT1 and PPARα,the key molecules of fatty acid β oxidation involved in ALR,were significantly decreased.2.The expression level of ALR protein in kidney tissue of DN patients was significantly increased,while the protein expressions of PPARα and CPT1,the key molecules of fatty acid β-oxidation involved in ALR,were significantly decreased.3.The distribution and expression levels of ALR,PPARα and CPT1 proteins in kidney tissue of DN patients showed consistent changes with those of DN mice. |
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