Correlation Between Polymorphism Of RTN4IP1 Gene Locus Rs3747790(T>C) With Coronary Heart Disease

Objective:To explore the correlation between reticulon 4 interacting protein 1(RTN4IP1)gene locus rs3747790(T>C)single nucleotide polymorphism(SNP)and coronary heart disease(CHD)susceptibility.Methods:187 patients with CHD who attended the Department of Cardiology of Yanbian University Hospital were selected as CHD group,and 189 physical examination subjects who excluded CHD were selected as the control group.The CHD group was compared with the control group by case-control method.Using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technology,the whole blood DNA extract of the study subjects was sequenced.SPSS26.0 software was used to analyze the data.Hardy-weinberg was used to analyze whether the genotype frequency had stable heritability,and the ratio of genotype distribution between CHD group and control group was analyzed byχ~2test.The relationship between the gene loci and the risk of CHD was analyzed by logistic regression model to calculate the odds ratio(OR)and 95%confidence interval(CI).Results:1.The detection rate of hypertension and diabetes was higher in the CHD group than in the control group.2.Genotype distribution of RTN4IP1 gene locus rs3747790(T>C)in CHD group and control group was conform to Hardy-Weinberg genetic equilibrium law.3.The distribution of each genotype in CHD group and control group was statistically different.4.Compared with TT genotype,CC genotype is a risk factor for CHD(OR=2.825,95%CI:1.343-5.943,P=0.006).5.Compared with the T allele,the C allele is a risk factor for CHD(OR=1.395,95%CI:1.033-1.885,P=0.030).6.Compared with CC genotype,CT+TT genotype is a protective factor for CHD(OR=0.369,95%CI:0.182-0.748,P=0.006).Conclusion:RTN4IP1 gene locus rs3747790(T>C)polymorphism is associated with susceptibility of CHD,and people carrying the C allele or CC genotype have an increased risk of CHD,CT+TT genotype is a protective factor for CHD.