Research Mechanism Of Ginsenoside Re On Protecting Human Umbilical Vein Endothelial Cells Induced By Lipopolysaccharide Through Rho/ROCK Pathway

Objective:To investigate the effects of ginsenoside Re(GS-Re)on the expression of RhoA,ROCK2,p-MLC and p-ERM in human umbilical vein endothelial cells(HUVECs)induced by lipopolysaccharide(LPS)in Rho/ROCK signaling pathway,and to provide a theoretical basis for the pharmacological treatment of cerebrovascular diseases caused by atherosclerosis.Methods:1.The cell viability of GS-Re(5-80μM)on HUVECs was detected by CCK8 method by using primary HUVECs cultured in vitro as experimental object.2.In this experiment,the cells were randomly divided into 4 groups:Normal control group,LPS injury group(100ng/ml),GS-Re low-dose group(GS-Re 20μM+LPS 100ng/ml),and GS-Re high-dose group(GS-Re 40μM+LPS 100ng/ml).3.RhoA,ROCK2,p-MLC and p-ERM protein level expression in HUVECs was detected by Western blot test.4.RhoA,ROCK2 and p-Ezr mRNA expression in HUVECs was detected by fluorescence quantitative PCR.Results:1.When the drug concentration of GS-Re was in the range of 5-80μM,there was no significant difference in the cell viability on HUVECs compared with the normal control group.2.Compared with the Control group,the expressions of RhoA,ROCK2 and p-ERM protein were significantly increased in the LPS injury group(P<0.001).Compared with the Control group,the expression of p-MLC protein in the LPS injury group was increased,but there was no significant difference.Compared with the LPS injury group,the protein expression of p-MLC in the low dose and high dose GS-Re groups was decreased,but there was no significant difference.Compared with the LPS injury group,the expressions of RhoA,ROCK2 and p-ERM protein were significantly decreased in the high dose GS-Re groups(P<0.05).3.Compared with the Control group,the mRNA level of RhoA,ROCK2 and p-Ezr in the LPS injury group were significantly up-regulated(P<0.05).Compared with the LPS injury group,the mRNA level of RhoA,ROCK2 in the low dose and high dose GS-Re groups were significantly down-regulated(P<0.05).Compared with the LPS injury group,the mRNA level of p-Ezr in the high dose GS-Re group was significantly down-regulated(P<0.01).Conclusions:Ginsenoside Re may protect HUVECs against LPS-induced injury by inhibiting the expression of RhoA,ROCK2 and p-ERM in the Rho/ROCK signaling pathway,which may be the mechanism of GS-Re in the treatment of atherosclerosis.