LncRNA SNHG1 Acts As A CeRNA To Facilitate The Progression Of Pancreatic Cancer

ObjectiveThis study aims to initially explore the biological function and mechanism of Long non-coding RNA small nucleolar RNA host gene 1(LncRNA SNHG1)in pancreatic cancer(PC),providing a new direction for the treatment of PC.Materials and MethodsThe role of SNHG1 on cells proliferation,migration,invasion,apoptosis and the epithelial-mesenchymal transition(EMT)was assessed by MTT,EDU,Wound healing,Transwell assays and Western Blot in vitro.Bioinformatics analysis,the dual-luciferase reporter assays,Western blot,qRT-PCR,and rescue assays were used to examine the regulatory mechanism of SNHG1.Finally,the growth of tumors was observed in vivo assays.ResultsDownregulation of SNHG1 blocked cells proliferation,migration,invasion,and induced apoptosis in vitro,while also inhibited the EMT,shown by an increase in the expression of E-cadherin(E-CAD),decrease of N-cadherin(N-CAD)and Vimentin(VIM).The opposite results were completely observed when overexpression of SNHG1.In vivo experiments showed that downregulation of SNHG1 inhibited tumor growth in nude mice.Rescue experiments demonstrated that the effects of SNHG1 downregulation on PC cells were attenuated when simultaneously inhibiting the levels of miR-497.Mechanistically,SNHG1 acted as a competing endogenous RNA(ceRNA),positively regulating the expression of Fibroblast growth factor receptor 1(FGFR1)through sponging miR-497 to promote the progression of PC.Conclusion1.SNHG1 promotes abnormal proliferation,invasive metastasis,and EMT of PC.Targeting blockade of SNHG1 negatively regulates the malignant biological behavior of PC.2.SNHG1 promotes the progression of PC by regulating FGFR1 expression via competitively binding to miR-497.