Screening And Identification Of Differential Proteins In Oral Squamous Cell Carcinoma Using 4D Label-free Quantitative Proteomics

ObjectiveTo explore the potential molecular mechanism of oral squamous cell carcinoma(OSCC),4D Label-free proteomics analysis combined with liquid chromatography-mass spectrometry was used.Lc-ms /MS were used to identify and analyze the differentiation of proteins in para-cancer and tumor tissues of OSCC patients,so as to screen out key differential proteins and enriched KEGG signaling pathways related to the pathogenesis of OSCC,and to identify their functions.MethodsAccording to inclusion and exclusion criteria,tumor tissues(experimental group)and paracancer tissues(control group)of 5 OSCC patients were selected for 4D Label-free protein group student letter analysis,so as to screen out differential proteins related to OSCC.The differential proteins were analyzed by gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).It was further verified by Western Blotting and IHC methods.Results4D Label-free proteomics analysis showed that 77 differential proteins(66 up-regulated proteins and 11 down-regulated proteins)were identified in this study.GO function and KEGG pathway enrichment analysis of differential proteins showed that the most significantly up-regulated expression of differential proteins in GO function were tyrosine protein phosphatase non-receptor 2 type(PTPN2)and Ra B-like protein 6(RABL6).KEGG enrichment analysis showed that differential proteins were enriched in cancer transcriptional dysregulation pathways.Werstern Blot results showed that PTPN2 was highly expressed in tumor tissues,and immunohistochemistry results showed that PTPN2 was more obvious in tumor tissues,while No PTPN2 nuclei were found in paracancer cells.ConclusionsIn this study,differential proteins were identified in tumor tissues and adjacent tissues of OSCC patients by unlabeled quantitative proteomics analysis,and all differential proteins were enriched and analyzed by GO and KEGG databases.Through the study,we screened the PTPN2 protein closely related to the pathogenesis of OSCC,and KEGG found that the pathways of differential protein enrichment were mainly metabolic pathways and cancer transcriptional dysregulation pathways.Furthermore,Werstern Blotting and IHC were used to verify the screened proteins,and the results suggested that PTPN2 was positively correlated with OSCC.The results of this study can provide molecular biological basis for the further exploration of OSCC targeted therapeutics.