Correlation Analysis For Ferroptosis Suppressor Protein 1 And Acute Myocardial Infarction Prognosis

Background and objectiveCoronary atherosclerotic heart disease(CHD)has gradually become one of the most common causes of heart damage.Although the diagnosis and treatment of coronary heart disease have made great progress in recent years,the morbidity is still increasing.And the mortality continues to rise.With the deepening of research,it is found that ferroptosis is closely related to the occurrence and development of CHD and acute myocardial infarction(AMI).The core of ferroptosis is the selenoenzyme glutathione peroxidase 4(GPX4),which catalyzes the reduction of polyunsaturated lipid hydroperoxides species to non-toxic hydroxy lipid species,thus preventing uncontrolled peroxidation of polyunsaturated fatty acids(PUFAs)and PUFAcontaining membrane phospholipids.The research found that the increase of GPX4 level can protect cardiac function after myocardial infarction.And ferroptosis suppressor protein 1(FSP1)is a newly discovered ferroptosis inhibitor.It has been identified to be able to complement GPX4 loss and participate in the regulation of reactive oxygen species(ROS)and promote mitochondrial electron transport.Its biological characteristics are associated with cardiovascular disease.Based on these biological characteristics,we speculate that FSP1 has a certain correlation with the occurrence of CHD and AMI.At present,there are few studies on the detection of FSP1 level.The purpose of this study was to investigate the relationship between FSP1 and CHD,AMI and their prognosis by detecting the level of FSP1.Materials and MethodsThe study collected a total of 150 patients who hospitalized the Department of Cardiology,Qianfoshan Hospital Affiliated to Shandong University from 2021-03-01 to 2021-10-31.Given the symptoms,sign and auxiliary examination results,patients were divided into non-CHD group(n=43),unstable angina pectoris(UAP)(n=63)and AMI(n=44).We collected blood samples after admission.The FSP1 level is determined by enzyme-linked immunosorbent assay.The B-type natriuretic peptide(BNP)level was detected by immunofluorescence assay.The cardiac Troponin I(cTn I)level was determined by chemiluminescence assay.The left ventricular ejection fraction(LVEF)was evaluated by biplane Simpon rule.The patients in the AMI group were followed up for 6 months and the occurrence of major adverse cardiovascular event(MACE)was recorded.We used SPSS 23.0 for statistical analysis.The differences level of FSP1 were evaluated using Student’s t-test and analysis of variance.Correlations were analyzed using Spearman or Pearson correlation.The potentially influential factors were analyzed via binary logistic regression.Results1.The expression level of FSP1 increased in AMI groupThe FSP1 level logarithmically transformed into ln-FSP1.There was a statistically significant difference(P<0.05)in ln-FSP1 level among non-CHD(5.36±0.51)and CHD group(5.36±0.51).When we divided all patients into the non-CHD group,UAP group and AMI group.The FSP1 levels among the non-CHD group and AMI group also has statistically significant difference.2.The FSP1 level has a positive correlation with cTn I and BNP,and negatively correlated with eGFRThe result of correlation analysis:ln-FSP1 level has a correlation with cTn I(r=0.163,P<0.05),BNP(r=0.171,P<0.05)and eGFR(r=-0.185,P<0.05).3.The FSP1 level has a positive correlation with ageThe result of ln-FSP1 level positively correlate with age(r=0.223,P<0.01).4.The expression level of FSP1 increased in the MACE groupWhen we divided AMI group into MACE group(431.4±245.6pg/ml)and non-MACE group(288.1±139.6pg/ml),the level between two group was statistically significant different(P<0.05).Univariable analysis indicated that FSP1 was a statistically risk predictor for MACE events.ConclusionThe expression of FSP1 is related to the occurrence and development of CHD and MACE after myocardial infarction.FSP1 can be used as biomarkers for the clinical diagnosis and prognosis of AMI.