Interventional Effects Of Yihe Tang And Curcumin On Thioacetamide-induced Liver Fibrosis In Larval Zebrafish

Objective: To use thioacetamide to induce liver lesions in zebrafish larvae,to create a liver fibrosis model,and to evaluate the liver area,fat deposition,fibrosis degree and glutathione content.Afterwards,the repairing effects of two liver-protecting drugs,Yihe decoction and curcumin,were respectively monitored,and their mechanism of action was discussed.Research methods: The larval zebrafish 2 d after exposure(2 dfp)were selected for exposure to 0.06% thioacetamide aqueous solution,and incubated for 6 d to build a liver fibrosis model.Then add Yihe decoction or curcumin treatment and incubate at28 ℃ incubator to 8 dpf(treatment 3 d),detect the changes of fibrosis-related gene transcription levels such as liver index and Tgf-β in zebrafish larvae,and stain with Sirius red.,glycogen staining,and detection of glutathione content to assess changes in protein levels.Research results: After 8 days of thioacetamide exposure,the fluorescence area of the liver decreased,and picric acid Sirius red staining showed significant collagen fiber deposition and glutathione content,while the Yihe decoction group or curcumin treatment significantly recovered.Changes in various indicators caused by thioacetamide.Thioacetamide exposure significantly increased the expression of fibrosis-related genes(Tgf-β,ACTA2,and Col1α1)(P<0.05),while Yihetang treatment(0.5mg/g+TAA)inhibited these genes The expression increased(P <0.05),and the curcumin treatment also showed a similar expression trend.The above results show that thioacetamide can make liver fibrosis model,and both Yihe decoction and curcumin can significantly inhibit liver fibrosis,which may be produced by inhibiting oxidative stress.