| Objective:In this study,the metabolomics technology based on ~1H NMR was used to analyze the changes of urine metabolites of rats in each group and to evaluate the intervention effect of Rubus Suavissimus S.Lee on T1DM.Methods:1.Thirty rats were randomly divided into 5 groups,namely the control group,the model group,Rubus Suavissimus S.Lee high-dose group(H-RS),Rubus Suavissimus S.Lee low-dose group,and the positive control group.Except for the control group,the other four groups were injected with large doses of streptozotocin to construct type 1 diabetes models.A blood glucose meter was used to measure blood glucose,and rats with fasting blood glucose>16.7 mmol/L in 3 consecutive trials were admitted as experimental subjects.After the model was built,the control group and the model group were given pure water by gavage,Rubus Suavissimus S.Lee low-dose group(H-RS),Rubus Suavissimus S.Lee high-dose group were given 3 g/kg and 6 g/kg(1ml/100 g)RS,respectively;and the positive control group received 200 mg/kg metformin hydrochloride.It is administered once a day for 6 weeks.At the same time we measured body weight,MDA and other indicators of each group to evaluate the therapeutic effect of RS on T1DM.2.Using ~1H NMR-based urine metabolomics technology combined with multivariate statistical analysis methods to find out potential biomarkers related to T1DM,and analyze the possible relationship between the potential biomarkers and T1DM,aiming to find out T1DM-related potential biomarkers and possible targets.In addition,we will further explore the changes of RS on endogenous metabolites in the urine of T1DM rats,and discover the potential biomarkers and possible targets related to the anti-T1DM effect point.Results:1.Compared with the control group,the model group had increased FBG,decreased weight,and increased oxidative stress levels in the body.After the intervention of RS,the FBG of T1DM rats decreased,the trend of weight loss slowed down,and the oxidative stress level was improved.2.Through metabolomics analysis,we determined that the levels of 9metabolites in T1DM rats had changed compared with control rats,namely lactate,acetate,pyruvate,succinate,2-oxoglutarate,citrate,creatinine,allantoin and hippurate.These metabolites are mainly related to metabolic pathway such as glycolysis/gluconeogenesis,pyruvate metabolism,and tricarboxylic acid cycle.After treatment with RS,the levels of these nine potential biomarkers have been significantly adjusted.Conclusion:RS can significantly improve the symptoms of type 1 diabetes,and RS may exert anti-diabetic effect in T1DM rats by synergistically regulating the three abnormal metabolic pathways of glycolysis/gluconeogenesis,pyruvate metabolism,and tricarboxylic acid cycle.These findings provide a reference for Rubus Suavissimus S.Lee in the treatment of diabetes,and also provide a theoretical basis for its development and use. |
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