Screening And Co-expression Of Ameloblastoma Invasion Related Genes,Long Non-Coding RNA And Micro-RNA

Objective: In this study,the second-generation high-throughput fulltranscription sequencing technology was used to screen and predict the Micro RNA(mi RNA),long non-coding RNA(lnc RNA)and their target genes related to invasion of Ameloblastoma(AB).Verify the differential expression and co-expression relationship by q PCR,and explore the key regulatory targets in the process of invasion.Methods:(1)A total of 16 pairs of fresh AB tissue and normal tissue adjacent to the tumor were collected and stored at-80°.The second-generation highthroughput full-transcription sequencing technology was used to sequence 4 AB tissues and 3 normal tissue.Through bioinformatics analysis,screening and prediction of mi RNA,lnc RNA and their target genes related to invasion of AB.(2)The differential expression of mi RNA,lnc RNA and their target genes in 16 pairs of tissues were verified by q PCR.(3)The age,gender,ethnicity,histopathological type,tumor volume and recurrence of the 16 patients were collected.We analyzed the relationship between differentially expressed mi RNA,lnc RNA and their target genes and clinicopathological characteristics by nonparametric test and spearman correlation.Results:(1)Through gene sequencing and bioinformatics analysis,we screened a total of 20 pairs of lnc RNA-m RNA regulation combinations,259 pairs of mi RNA-m RNA regulation combinations,and 8 pairs of mi RNA-lnc RNAm RNA regulatory combinations.(2)After q PCR detection,the relative expression of m R-SPRY4 in AB tissue is higher than that in adjacent tissues,significant difference statistically(P=0.014,non-parametric test);the relative expression of lnc R-SPRY4-AS1 in AB tissue is higher than that in adjacent tissues,significant difference statistically(P=0.002,non-parametric test);there was no significant difference in the expression of mi R-4510 in AB tissues and adjacent tissues(P=0.926,non-parametric test).(3)The relative expression of lnc R-SPRY4-AS1 and m R-SPRY4 in AB has nothing to do with age,ethnicity,histopathological type,recurrence and tumor size.Conclusion:This study screened and verified the lnc R-SPRY4-AS1 and its target gene SPRY4 that may be related to AB invasion through high-throughput sequencing,biometric analysis and q PCR,suggesting that the co-expression relationship between lnc R-SPRY4-AS1 and m R-SPRY4 may be exist.These genes are the first reports in AB,which provide reliable targets for the study of AB invasion mechanism and have important clinical practical value.