Humanized Mice Model Established By Transplanting Gut Microbiota From Longevity People And Its Effects On Aging Related Phenotypes

The interactions between gut microbiota and hosts have a great influence on hosts’ health.There is growing evidence to suggest that a close relationship exists between gut microbiota and aging.Long-living people have been regarded as a suitable model for studying healthy aging and gut microbiota due to their distinctive microbiota structure with ordinary old’s and lower morbidity of senile diseases.However,the relationship between the microbiota of long-living people and aging phenotype remains largely unknown.Herein,this study established humanized mice by transplanted feces of long-living people and that of ordinary old to mice respectively,which were examined their aging related indexes and using16 S r RNA high-throughput sequence analyzed changes and differences in structure of gut microbiota between two groups in order to find out the relationship between long-living people’s gut microbiota and aging.The main results of this study are showed as following:1.Antibiotic treatment changed the predominant phylum of mice from Bacteroidetes and Firmicutes to Proteobacteria and reduced their alpha diversity effectively but no significant effect on weight.And the proportion of humanized in mice reached the peak at G2 and reduced with time.Dissimilarity of microbiota between recipents at S12 and their donors were significant lower than that between recipents at S12 and at Ab0.2.Mice transplanted with fecal matter from long-living people(L group)accumulated less β-galactosidase in their hearts and ileum and less lipofuscin in their brain than mice transplanted with fecal matter from ordinary old(E group).L group also had longer intestinal villi,while there were no significant between group E and L in SOD activity,GSH-PX activity and MDA concentration in plasma.3.The alpha diversity of L group were significant higher than that of E group at G2.Also,L group had higher humanized proportion than E group.4.L group had more probiotic genera(Lactobacillus,Bifidobacterium),and butyrate producing genera(Oscillospira,Anaerofustis,Roseburia,Faecalibacterium,Ruminococcus,Coprococcus)than that in E group and less Opportunistic pathogen like Bilophila wadsworthia.In summary,by using long-living people as an ideal model to study the relationship between healthy aging and gut microbiota,our study have found that the gut microbiota from long-living people can anti-aging,which indicates the effects that the gut microbiota from long-living people have on healthy aging.