The Effect Of Human IPS Cells On The Progression And Metastasis Of Human Breast Cancer Cells

Induced pluripotent stem cells(iPS)are a class of stem cells that can proliferate indefinitely and can differentiate into a variety of somatic cells.These characteristics are similar to those of embryonic stem cells(ESC),and IPscs are free from ethical problems and limitations of immune rejection.Stem cells have been widely used for research and treatment in many fields such as clinical medicine and biology,but there are still some safety problems in the process of cancer treatment with stem cell therapy.Therefore,it is of certain research significance to explore the interaction mechanism between iPS and tumor cells.As for whether stem cells can play a role in the growth and metastasis of tumor cells,a large number of studies have been carried out in the academic community.Some experimental studies have shown that stem cells can make the metastasis of cancer cells better,but some studies have reported that there is no adverse prognosis associated with stem cell therapy.Therefore,this paper focuses on the study of the effect of human iPS on MDA-MB-231 cells and T-47D cells in the process of epithelial-mesenchymal transformation(EMT)in human triple-negative breast cancer.In this paper,the expression of EMT related genes needs to be investigated and the fluorescence quantitative data need to be analyzed,so we preliminarily proposed a method that can be automatically analyzed according to the fluorescence quantitative PCR data,which is briefly introduced in this paper as a by-product of the paper.Research objective:The effects of iPS on EMT processes in human MDA-MB231 cells and T-47D cells of human ductus mammary carcinoma.Research methods:The culture conditions of human MDA-MB-231 cells and human ductus carcinoma T-47D cells and human iPS cells were optimized to maintain good growth status.Transwell chamber was used to co-culture MDA-MB-231 cells,T47D cells and iPS indirectly.The small molecules secreted by human iPS could penetrate into the microenvironment of MDA-MB-231 cells and T-47D cells of human breast ductal carcinoma through the membrane.To study the differences in invasion ability,proliferation,EMT-related genes MMP-2,MMP-9,Snail,Slug,and Twist mRNA expression levels of human MDA-MB-231 cells and human ductal carcinoma T-47D cells in co-culture with indirect contact with human iPS.Research resultand conclusion:(1)After co-culture with human iPS,the invasion ability and proliferation ability of MDA-MB-231 cells and T-47D cells of human ductal carcinoma in the experimental group were enhanced compared with those in the control group.The expression levels of MMP-9 in the experimental group and MDA-MB-231 cells and T-47D cells of human ductal carcinoma of the breast were 4.71 and 7.19 times higher than those in the control group,respectively.The expression levels of MMP-2 in the experimental group and the human ductal carcinoma T-47D cells were 2.48 and 5.62 times higher than those in the control group,respectively.The expression levels of MMP-2 and MMP-9 were significantly increased RNA expression levels of Snail,Slug and Twist were also slightly up-regulated.(2)The result show that human induced pluripotent stem cells with some human breast cancer MDA-MB-231 and T-47D cells after co-culture can promote epithelialmesenchymal transition,enhance invasion and proliferation ability.