The Influence Of Circular RNA Hsa＿circ＿0008202 On The Biological Characteristics In Oral Carcinoma Cell Lines

【Objective】The aim of this research is to explore the effect of circular RNA hsa＿circ＿0008202 on oral carcinoma cell lines,detect its role in the development and occurrence of oral squamous cell carcinomas(OSCC)in cell proliferation,apoptosis,migration,invasion,and discuss the possible mechanism.【Method】In our study,the Quantitative real time-Polymerase chain reaction(q RT-PCR)was used to detect the expression of circular RNA hsa＿circ＿0008202 in the four oral carcinoma cell lines,and the two lowest will be chosen for later experiments.To enhance the expression of hsa＿circ＿0008202,we design overexpressed plasmid pc DNA-8202 and transfected it into oral carcinoma cells.The empty vector was choosen as the negative control group.The transfection efficiency was detected by RT-PCR.Proliferative capacity of overexpressed cells and control cells was detected by Cell Counting Kit-8(CCK-8)and EDU Cell Proliferation&Viability Assay Kit.Migration and invasion of the two group cells was tested by wound healing assay and Transwell migration/invasion assay.The Hoechst staining assay and Annexin V-FITC/PI double marker flow cytometry were applied to measure the apoptosis level in the two group cells.The expression of related proteins was detected by Western blotting analysis.【Result】The expression of circular RNA hsa＿circ＿0008202 in SCC15 and CAL27are lower than other oral carcinoma cells detced by RT-PCR(~*P<0.05).The overexpressed plasmid pc DNA-8202 and empty vector were successfully transfected into SCC15 and CAL27 cells.Compared with the empty vector group,the expression in pc DNA-8202 group were increased significantly(~*P<0.05).CCK-8 and EDU Cell Proliferation&Viability Assay results showed cells proliferative capacity and cells viability in pc DNA-8202 group were down-regulated compared to empty vector group(~*P<0.05).Wound healing assay and Transwell migration analysis results showed that migration rate in pc DNA-8202 group were decreased in comparison with the empty vector group(~*P<0.05).Transwell invasion assays result showed invasion rate in pc DNA-8202 group were decreased in comparison with the empty vector group(*P<0.05).The Hoechst staining assay and Annexin V-FITC/PI double marker flow cytometry results showed higher apoptosis rate in pc DNA-8202 group were decreased compared with the empty vector group.As the result of Western blot,Bcl-2and MMP-9 were down-regulated whereas Bax was up-regulated in pc DNA-8202group compared to the empty vector group.【Conlusion】After hsa＿circ＿0008202 up-regulated in oral carcinoma cells,the cells proliferative capacity and cells viability were descended,the cells apoptosis level was ascended.Furthermore,cells migration rate and invasion rate were decreased.Circular RNA hsa＿circ＿0008202 may play an necessary role in the inhibition of oral squamous cell carcinoma genesis and development.