| Parkinson disease(PD)is a neurological degenerative disease characterized by dopamine(DA)neurons degeneration,loss,and formation of Lewy bodies(Lewy).The main clinical manifestations are tremor,Dyskinesias such as muscle rigidity,bradykinesia,and abnormal posture gait.The incidence of this disease is high and as the disease progresses,multiple systems can be accumulated,which seriously affects the quality of life of patients and also causes a great burden on society.At present,western medicine therapy is the main method of treating Parkinson’s disease,but medicine cannot cure the disease fundamentally,and it brings serious adverse reactions and side effects.Surgical treatment,as an adjuvant to medicine,is expensive and slow to take effect.Moxibustion is an important traditional non-drug therapy in China.In recent years,moxibustion has been more and more used in the clinical treatment of PD,but there are relatively few researches on related functions and mechanisms.ObjectiveThis study intends to take Parkinson’s model rats as the research object,cut through the oxidative stress injury and Nrf2/ARE pathway,and systematically explain the antioxidative damage effect and mechanism of moxibustion in the treatment of PD,which could provide experimental basis for clinical application of moxibustion.MethodsMale SD rats were randomly divided into normal group(n=12),sham operation group(n=12),model group(n=12)and moxibustion group(n=12).The lateral PD model was established by injection of 6-hydroxydopamineinto the right substantia nigra and the ventral tegmental area of the midbrain,with sham operation group injected with the same amount of normal saline.Moxibust ion was applied to‘Baihui’and‘Sishencong’for 20 min once a day,6 days for a course of treatment,interval of one day between each treatment treatment,and 6 courses of treatment totally;without any treatment for the other groups.After the experiment,to observe the behavioral changes of rats in each group.The pathomorphism change of nerve cell of right midbrain nigra was detected by HE staining and the immunohistochemical technique was used to detect the TH positive neurons.The ROS,MDA,GSH content and GSH-Px activity in the striatum of rats were determined by chemical colorimetry,the Nrf2 and HO-1 expression determined by Western blot,and the mRNA expression was determined by Real-time RCR method.Results1.The rats in the normal group and the sham operation group did not show rotation behavior throughout the experiment.Rats in the PD group exhibited rotation behavior,and there was no significant difference in the number of rotations after treatment.Compared with the model group,the number of rotations induced by APO in the moxibustion group was significantly reduced after treatment(P<0.05).2.Compared with the control group,the neuronal structure of rats in the PD group was chaotic,the number of DA neurons and TH expression were significantly decreased.The neuronal damage in the moxibustion group was reduced,DA neurons were partially recovered,and TH expression was increased.3.Compared with the control group,the ROS and MDA contents in the model group were significantly increased,while the GSH and GSH-Px values were reduced,and the expressions of Nrf2 and HO-1 were reduced.Compared with the model group,ROS and MDA in the moxibustion group were significantly reduced,GSH and GSH-Px values were significantly increased,and the expressions of Nrf2 and HO-1 were increased.ConclusionMoxibustion has a therapeutic effect on PD model rats,can improve its behavior,reduce oxidative stress injury,protect original DA neurons in brain tissue and increase TH expression,which may be related to the activation of Nrf2 signaling pathway. |
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