| Objective1.Exploring an new detection methods for predicting carotid unstable plaques through analyzing the proteomic differences between healthy people and patients with atherosclerotic carotid unstable plaques,and;2.To explore the biomarkers that can be used to early identify high-risk populations with carotid unstable plaques or arterial stenosis by analyzing proteomic differences at different stages of atherosclerosis.3.Exploring the proteomic differences of different syndromes in order to provide experimental evidence for the study of Phlegm dampness syndrome and Phlegm Stasis syndrome.MethodsThe plasma samples were taken from subjects in a prior randomized double-blind placebo-controlled clinical trial.Grouped according to atherosclerosis process,based on carotid color Doppler ultrasound and angiography results,All the samples were divided into control group(C group),stable plaque group(SP group),unstable plaque group(UP group)and arterial stenosis group(S group).Using antibody microarrays to detect the protein profile of each group of plasma samples.Observation indicators include:(1)protein expression among four groups of patients grouped according to the atherosclerosis process;(2)protein expression of different TCM syndromes.ResultsA total of 184 subjects who met the inclusion and exclusion criteria were included in this study.184 eligible plasma samples were collected and completed proteomics testing.Protein detection was performed on all plasma samples using an 187 AS-associated target protein antibody microarrays.Using R software statistical analysis,it was found that among the differential proteins in group C and the other three groups,11 co-expressed down-regulated proteins and 30 co-expressed up-regulated proteins.When using Mev software for selection analysis,59 differentially expressed proteins were selected during the development process of C→SP→UP→S,of which 28 were differentially expressed proteins that were up-regulated and 31 differentially expressed proteins that were down-regulated.Based on the selection results of the two methods,there are 37 up-regulated differential proteins and 33 down-regulated differentially expressed proteins between the control group and the atherosclerosis group,which can be used as potential organisms for carotid unstable plaques and carotid stenosis.Among them,there are 6 most significant proteins(P<0.001),namely Aromatic hydrocarbon receptor,Cathelicidin antibacterial peptide,Cartilage oligomeric matrix protein,Catenin β-1,Vitamin D binding protein and Tissue factor pathway inhibitor.The signal pathway enrichment analysis was performed using the KEGG database.During the C→SP→UP→S development process,the signal pathway was mainly up-regulated.This process has 12 co-enriched signal pathways,which are mainly related to inflammatory reactions and AS plaque formation and composition changes are related.According to the TCM syndrome group analysis,15 proteins with significantly different expressions related to phlegm and stasis syndrome were found(P<0.01),and 3 differential proteins related to phlegm and dampness syndrome were found(P<0.01),which are mainly involved in biological processes such as inflammatory response,coagulation,endothelial function,and lipid metabolism.It can provide potential biological evidence for TCM syndromes.ConclusionThe antibody microarrays prepared in this study contains 187 atherosclerosis-related proteins,which can be used as a new detection method for predicting carotid unstable plaque.The research found that there were multiple differentially expressed proteins related to atherosclerotic unstable plaques,arterial stenosis,and Phlegm dampness syndrome,Phlegm stasis syndrome,all of which were potential diagnostic biomarkers.At the same time,it can provide potential evidence for the biological basis of TCM syndromes. |
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