A Study Of The Anti-Cancer Effect And Molecular Mechanism Of Fig Extract In Human Hepatoma Cell Line SMMC-7721

Objects: This study took hepatoma cell-SMMC-7721 as the research object.Cell morphology,cell count,MTS method,scratch test,Western bolt were used to observe fig extract(FE)on human liver cancer cell growth inhibition and apoptosis induction effect.Methods: Human hepatoma cell-SMMC-7721 was cultured in vitro,and logarithmic growth phase cells were used for experiments.The FE was diluted with RPMI.1640 incomplete medium into low concentration group(1.25ul/ml),medium concentration group(2.5ul/ml),high concentration group(5ul/ml),and 75% alcohol was diluted with RPMI.1640 incomplete medium into negative control group(5ul/ml).The morphological changes of cells in different concentration groups were observed by ordinary light microscopy after 6h,12 h,24h,36 h and 48 h,and the number of adherent cells was calculated under microscope.MTS method was used to detect different concentration groups of FE on the effects of the survival rate of SMMC-7721 cells before and after treatment for 12 h,24h and 48 h.Scratch test was used to detect the effect of FE before and after treatment on the migration ability of SMMC-7721 cells in different concentrations.The expression of Caspase-3 protein in SMMC-7721 cells before and after FE treatment in different concentrations was detected by Western blot.Results: With the increase of FE concentration and prolonged action time,SMMC-7721 cells gradually deteriorated,cell gap gradually increased,cell vacuoled,decellularized cells increased,adherent cells decreased,and cell debris increased under microscope.There was no significant difference between the negative control group and the control group(P>0.05).There were significant differences between the different concentrations of FE concentration group and the control group at different time points(P<0.05).The results of MTS showed that the cell survival rate after FE treatment was significantly lower than the control group(P<0.05).There was no significant difference between the negative control group and the control group(P>0.05).The cell survival rate was within the scope of the study and the drug effect.Concentration and duration of action were negatively correlated.The results of scratch test showed that the cell migration area after FE treatment was significantly lower than the control group(P<0.05).There was no significant difference in cell migration between the negative control group and the control group(P>0.05).The results of Western bolt showed that the expression of Caspase-3 protein increased with the increase of FE concentration,but no expression of Caspase-3 protein in the control group and the negative control group.Conclusions: Fig extract significantly inhibited the growth of SMMC-7721 cells.The inhibitory effect was positively correlated with FE concentration and duration of action.The extract of fig extract induced SMMC-7721 cell apoptosis,and its apoptosis-inducing mechanism may involve Caspase-dependent Apoptotic signal regulatory pathway.