| Objective Under the guidance of Traditional Chinese Medicine theory,Using molecular biology,gene expression and other modern scientific and technological means to detect the biochemical indexes,pathological changes of liver tissue and the geneexpression lever of TLR4,NF-κB,TNF-α in liver tissue of rats model of NAFLD intervented by different alcohol consumption.To analyze the effects of differe-nt alcohol consumption on the TLR4/NF-κB signaling pathway in NAFLD model rats and to study the mechanism of its impact.To explore the pathogenesis of NAFLD so as to provide safe drinking instruction for clinical drinking patients,and provide reference for NAFLD treatment.Methods 40 healthy male SD rats were randomly divided into normal control group(n=10)with ordinary feeding;model control group,moderate alcohol consumption group,and excessive drinking group(n=10),all with high fat diet feeding.After eighth weeks of experiment,the NAFLD models were established,then the rats were given different interventions that the moderate alcohol consumption group rats were gavaged by 2.0g/kg/d dose of spirit and excessive drinking group rats gavaged by 4.0g/kg/d dose of spirit,the normal group and the model group were given the capacity of the 0.9%normal saline.Each group rats were fed for 16 weeks,drinking water freely.At the end of the 16 week,the rats were killed,weighting body and liver wet weight and calculating liver index.Blood was collected and serum biochemical indexes were detected.The pathological changes of liver tissue were observed under microscope and the degree of liver steatosis and inflammation were calculated.PT-PCR detect the expression of TLR4/NF-κB gene in liver tissue.Results The terminal weight and liver index:the rats in moderate alcohol consumpti on group were significantly lower than those in model group and excessive drinking g roup(p<0.01).There was no significant difference between the excessive drinking grou p(P>0.05).Serum liver function:ALT,AST of the rats in moderate alcohol consumption group were decreased than the model group and excessive drinking group(p<0.05).The excessive drinking group was higher than the model group(P<0.01).Lipid level:C ompared with the model group,TC and TG were significantly lower(p<0.01)and HD L-C were higer(p<0.01)in moderate alcohol consumption group.The levels of TC an d LDL-C were significantly higher(P<0.01),and the levels of HDL-C and TG were not significantly different(P>0.05)in the excessive drinking group.Compared with the excessive drinking group,TC,TG,HDL-C and LDL-C were decreased in moderate alc ohol drinking group(P<0.01).Pathomorphology:Compared with the model group,the de gree of steatosis and inflammation in moderate alcohol consumption group were signifi cantly reduced(p<0.01),There was no significant difference between the excessive dri nking group(P>0.05).TLR4/NF-κB gene expression level:The expression of TLR4 and NF-κB in liver tissue of moderate alcohol drinking group was lower than that of mod el group(p<0.01),while the expression level of TNF-α was decreased,but there was no significant difference(p>0.05).There was no significant difference between the ex cessive drinking group and the model group(P>0.05).Conclusion TLR4/NF-κB signaling pathway is closely related to the pathogenesis of NAFLD.Different alcohol consumption had different effects on NAFLD rats.Moderate drinking can significantly improve lipid metabolism and hepatic steatosis of NAFLD ra ts,improve liver function,repair liver damage,reduce inflammation and fibrosis of the liver.The expression of TLR4/NF-κB signaling pathway in liver tissue may be reduc ed,and the degree of hepatic steatosis and inflammation can be alleviated,and the pr ocess of fibrosis can be delayed or prevented,which can protect the NAFLD induced by high fat diet.Excessive drinking may aggravate liver damage and accelerate the de velopment of NAFLD rats.Combined with clinical,it can provide safe drinking guidan ce for patients with alcohol and provide reference for NAFLD. |
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