Genome-wide Analysis Of SPL Gene Family And Functional Identification Of GmSPL13A In Soybean

Flowering time is a key agronomic trait that has an important impact on plant yield and quality.In general,each plant has an optimal flowering time,and the process is induced by several factors such as photoperiod,growth years and hormones.The SPL gene family mainly regulates the growth and development of plants such as flowering by participating in embryonic development,seedling to adult transformation and organ development,and plays a key role in it.In this paper,we analyzed the members of the SPL gene family in soybean,specifically the GmSPL13 A gene,and based on the results of the analysis,we conducted functional validation and transcriptional regulation of the gene to determine the effect of the gene on flowering in soybean.(1)Total 43 soybean SPL gene family members containing complete SBP structural domains were successfully screened by bioinformatics analysis;and most of them belonged to basic proteins with subcellular localization predicted to be located in the nucleus;phylogenetic and covariance analysis revealed a large homology with Arabidopsis SPL gene family members.(2)The biological function analysis of GmSPL13 A gene was performed,including functional annotation,gene structural domain prediction,protein tertiary structure prediction,protein transmembrane structure and signal peptide analysis,promoter region analysis and expression location prediction.The gene was found to be expressed mainly in the apical meristem tissue of soybean stems,and combined with the analysis of cis-acting elements,the gene was found to be involved in the growth and development process of soybean.(3)The expression level of GmSPL13 A gene reached the highest level in the stem apical meristem tissue under long daylight conditions;and the expression of this gene was higher after two hours of light.(4)The analysis of GmSPL13 A gene and flowering-related gene expression in Gmspl13 A mutant and Willams82 control materials revealed that there was some kind of mutual regulation between GmSPL13 A gene and TOE5 a,LHY,LFY,AP1 and other genes.The expression of GmSPL13 A gene and its homologs in E1/e1,E2/e2,E2/e3 and E4/e4 materials were analyzed to clarify the mutual regulatory relationship between GmSPL13 A gene and E gene.GmSPL13 A is a new soybean flowering gene that is involved in the process of plant flowering in the regulatory pathway of soybean flowering,and mutations in this gene can lead to earlier flowering and affect soybean yield.The present study clarifies that the GmSPL13 A gene affects soybean flowering,which not only provides a research basis for supplementing the regulatory network of soybean flowering,but also provides an important molecular basis for breeding soybean for high yield,which is of great significance for agricultural production and theoretical research.