The Effects Of UVB Radiation On The Mantle Tissue Of Pacific Oyster Crassostrea Gigas

Sunlight is an important environmental factor for aquatic ecosystems.The photosensitive Crassostrea gigas,whether wild or cultured,may be affected by light throughout their life history,especially UVB radiation.This study comprehensively analyzed the physiological activity of C.gigas under UVB radiation through a combination of phenotype,behavior,physiology,molecular,and multiomics approaches,with a focus on key transcription factors affected by UVB radiation.Through validation methods such as co-immunoprecipitation(CO-IP)and dual luciferase reporter assays,the functional analysis of these key transcription factors was thoroughly investigated.The research findings are as follows:1.Analysis of the effects of UVB radiation on tissue damage,behavior,and related physiological indicators in C.gigasIn this study,C.gigas larvae were exposed to simulated PAR light with varying intensities of UVB.Following exposure,observation of the shell morphology revealed the formation of a new calcification layer on the inner surface of the C.gigas shell.The results of HE staining showed varying degrees of pathological morphological changes in the UVB treatment group.The physiological index measurements indicated that UVB radiation may induce cellular biological processes,such as oxidative stress,autophagy,and cell apoptosis.2.Multiomics integration analysis reveals the functional regulation network of C.gigas response to UVB radiationIn this study,an integrated analysis of full-length transcriptome,proteome and metabolome omics data was used through a combination of model analysis(x MWAS)and functional mapping(KEGG,GO functional enrichment).The results showed that UVB radiation negatively affected various material metabolic processes in the mantle tissue of C.gigas,such as energy metabolism,nucleotide metabolism,and protein metabolism,and caused disorder in many cellular biological functions such as oxidative stress,signal transduction,cytoskeleton,and biomineralization.3.Functional verification of key molecules in C.gigas responding to UVB radiationPredictive analysis of transcription factors that changed after exposure to simulated UVB light identified two upregulated transcription factors: Cg Runx1 and Cg CBFβ.Tissue expression analysis revealed that both genes were expressed at the highest level in the mantle tissue.Co-IP showed that these two factors could form heterodimers and interact with each other.Through screening of downstream target genes regulated by Cg Runx1 and prediction of binding sites,as well as dual luciferase reporter assays,it was verified that C.gigas may activate the transcription of Transport and Golgi organization 1 through Cg Runx1 and subsequently regulate their calcification process under UVB radiation.In summary,this study has deeply explored the effects of simulated sunlight containing UVB on the main physiological processes of C.gigas,proposed hypotheses regarding the mechanism of C.gigas response to light,and validated the effects of key transcription factors affected by light on downstream physiological functions.This research provides a foundation for exploring the photoreception mechanisms of visual-insensitive bivalves and a guideline for coping with UVB-driven environmental stress in aquaculture.