Mechanism Research Of Transporter Particle Complex Ⅲ Subunit MoTrs85 Regulates Autophagy In Magnaporthe Oryzae

Rice blast caused by Magnaporthe oryzae infection is one of the most devastating rice fungal diseases,which is a threat to the safe production of rice.Autophagy plays an important role in the infection of M.oryzae.Therefore,the study of autophagy and its regulatory mechanism has become a hot topic in plant pathology.Previous studies have shown that Autophagy is coupled with vesicle transport.In addition to autophagy related genes(ATGs)involved in autophagy,Rab proteins involved in vesicle transport are also involved in autophagy.guanine nucleotide-exchange factors(GEFs)regulate the activity of Rab proteins.In view of this,the study of GFEs in M.oryzae is helpful to understand the autophagy process.Transport protein particle(TRAPP)complex is a multisubunit tether complex,which is the GEF of Rab protein.Yeast TRAPP III complex is a GEFs of Ypt1(Rab1homologous),and its specific subunit Trs85 plays an important role in GEFs function and autophagy.However,Trs85 have not been reported in plant pathogenic fungi.We identified the homologous protein Mo TRS85 from M.oryzae and obtained the deletion mutant ΔMotrs85.Phenotypic analysis showed that the deletion of ΔMotrs85caused decreased growth rate,conidiation,abnormal septum development,decreased appressorium formation rate,delayed utilize of glycogen and lipid,defects in vesicle transport and endocytosis,and decreased pathogenicity of M.oryzae.Further showed that Mo TRS85 was involved in regulating the activation and stability of Mo Ypt1.Compared with wild-type strains,ΔMotrs85 resulted in the obvious fluorescence signal and protein expression of GFP-Mo Ypt1.In order to reveal the molecular mechanism of MoTrs85 regulating biological function of M.oryzae,the interaction between Mo Snx41 and MoTrs85 was found through CO-IP,bioinformation analysis and interaction verification.It has been reported that Mo Snx41 is involved in endosomal transport and peroxisome autophagy.In view of this,we speculate that Mo Snx41 and MoTrs85 interact and coregulate membrane transport.In conclusion,the results of this study reveal the molecular mechanism of MoTrs85 regulating autophagy mediated disease of M.oryzae,and provide theoretical basis for the development of environmentally friendly fungicides.