Exploration Of The Molecular Mechanism Of Tomato Response To Tomato Chlorosis Virus And Bemisia Tabaci Based On RNA-seq

Tomato chlorosis virus(ToCV)is one of the most destructive plant viruses in cultivated tomatoes,seriously threatening the growth and yield of tomato,and the spread of the virus and its hosts are still increasing.As the main insect vector for the rapid spread of ToCV,Bemisia tabaci(MED)Q has become the dominant population in our country.At present,the control of ToCV can only be achieved by spraying chemical agents,and there is no ToCV-resistant tomato variety on the market.Therefore,clarifying the response mechanism of tomato under ToCV and Bemisia tabaci infestation is conducive to the prevention and control of ToCV.We adopted nextgeneration sequencing to decipher miRNAs and mRNAs that are differentially expressed under the infection of B.tabaci and ToCV in tomato plants.Our results selected genes that may play a key defense role in the interaction of tomato-ToCV-B.tabaci.RT-qPCR was used to verify the sequencing results of randomly selected differentially expressed genes.The main findings are as follows:1.Both non-viruliferous and viruliferous B.tabaci feeding could significantly change the phytohormone content in tomato,and the difference between salicylic acid and jasmonic acid in tomato reached the maximum after B.tabaci feeding for 24 h.At the same time,the activities of antioxidant enzymes(CAT,SOD and POD)in tomato were higher than in control plants.2.A total of 6199 mRNAs were differentially regulated in tomato leaves under the infestation of ToCV and B.tabaci alone or together,and the differential genes were mainly enriched in plant-pathogen interaction,MAPK signaling pathway,and photosynthesis,the defense pathway of tomato was significantly activated under the action of virus.3.A total of 242 miRNAs were differentially regulated in tomato leaves under the infestation of ToCV and B.tabaci alone or together,and there were more differential miRNAs in the groups with ToCV involvement.The differential genes were mainly enriched in ABC transporters,basal transcription factor and substance metabolism pathway.4.Combined analysis of mRNA and miRNA revealed miRNA-mRNA pairs that play a key role in the response to ToCV and B.tabaci,and the regulatory network map revealed that solyc02g070393.1,solyc02g084890.2,solyc08g013970.2 and solyc08g007250.2 were associated with multiple target genes interaction,suggesting that they may play an important role in mediating plant disease resistance pathways.Taken together,the analysis reported in this article lays a solid foundation for further research on the interaction between tomato,ToCV and B.tabaci,and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.